2. Academic Validation
  3. Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress

Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress

  • Neurosci Res. 2021 May:166:55-61. doi: 10.1016/j.neures.2020.05.009.
Xiaowen Shi 1,  Yasuyuki Ohta 1,  Yumiko Nakano 1,  Xia Liu 1,  Koh Tadokoro 1,  Tian Feng 1,  Emi Nomura 1,  Keiichiro Tsunoda 1,  Ryo Sasaki 1,  Namiko Matsumoto 1,  Yosuke Osakada 1,  Yuting Bian 1,  Zhihong Bian 1,  Yoshio Omote 1,  Mami Takemoto 1,  Nozomi Hishikawa 1,  Toru Yamashita 1,  Koji Abe 2
Affiliations

Affiliations

  • 1 Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikatacho, Kitaku, Okayama 700-8558, Japan.
  • 2 Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikatacho, Kitaku, Okayama 700-8558, Japan. Electronic address: a215621091@gmail.com.
PMID: 32461139 DOI: 10.1016/j.neures.2020.05.009
Abstract

Cu-diacetyl-bis (N4-methylthiosemicarbazone) (CuATSM) has both anti-oxidative and anti-inflammatory activities, but its therapeutic efficacy for oxidative stress has not been thoroughly investigated in acute ischemic stroke. Here, the present study was designed to assess the efficacies of CuATSM in acute ischemic stroke by comparing with the standard neuroprotective reagent edaravone. Mice were subjected to transient middle cerebral occlusion (tMCAO) for 60 min, and then intravenously administrated with CuATSM (1.5 mg/kg) or edaravone (3 mg/kg) just after the reperfusion, and examined at 1 and 3 d. Compared with the vehicle group, CuATSM treatment decreased infarct volumes and oxidative stress at 3d after tMCAO, which was further enhanced by combined CuATSM + edaravone treatment as compared with single CuATSM group, but not improve neurobehaviors. The present study demonstrated that CuATSM showed strong antioxidative and neuroprotective effects in acute ischemic stroke, which was enhanced by the combination with edaravone.

Keywords

CuATSM; Edaravone; Oxidative stress; Stroke; Transient middle cerebral artery occlusion.

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