2. Academic Validation
  3. Biological applications of a turn-on bioluminescent probe for monitoring sulfite oxidase deficiency in vivo

Biological applications of a turn-on bioluminescent probe for monitoring sulfite oxidase deficiency in vivo

  • Eur J Med Chem. 2020 Aug 15;200:112476. doi: 10.1016/j.ejmech.2020.112476.
Xi Yang 1,  Ping Feng 2,  Lin Ma 3,  Ting Kang 4,  Shilong Hu 3,  Ao Hai 4,  Bowen Ke 4,  Jin Liu 5,  Minyong Li 6
Affiliations

Affiliations

  • 1 Laboratory of Anesthesia and Critical Care Medicine, Department of Anesthesiology, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China; Department of Anesthesiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, 610072, China.
  • 2 Institute of Clinical Trials, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
  • 3 Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China.
  • 4 Laboratory of Anesthesia and Critical Care Medicine, Department of Anesthesiology, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
  • 5 Laboratory of Anesthesia and Critical Care Medicine, Department of Anesthesiology, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. Electronic address: scujinliu@gmail.com.
  • 6 Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China. Electronic address: mli@sdu.edu.cn.
PMID: 32492597 DOI: 10.1016/j.ejmech.2020.112476
Abstract

Sulfites are widely used as preservative and antioxidant additives in food and drug. A non-invasive method for in vivo imaging of sulfite represents a powerful tool for estimating its potential effects in living organisms. Herein, we report the design, development, and application of sulfite bioluminescent probes (SBPs) for the analyte-specific detection of sulfite through sulfite-mediated intramolecular cleavage. Among them, SBP-1 exhibited the excellent responsivity, high selectivity and sensitivity. By taking advantage of this probe, the first in vivo imaging of sulfate was successfully carried out, not only to trace exogenous sulfite level in living animal, but also to investigate endogenous sulfite in a sulfite oxidase deficiency model.

Keywords

Bioluminescent probes; In vivo imaging; Real-time monitoring; Sulfite oxidase deficiency; Sulfites.

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