2. Academic Validation
  3. Maresin 1, a specialized proresolving mediator, stimulates intracellular [Ca2+ ] and secretion in conjunctival goblet cells

Maresin 1, a specialized proresolving mediator, stimulates intracellular [Ca2+ ] and secretion in conjunctival goblet cells

  • J Cell Physiol. 2021 Jan;236(1):340-353. doi: 10.1002/jcp.29846.
Markus V Olsen 1 2 3 4 5,  Anne V Lyngstadaas 1 2 3 4 5,  Jeffrey A Bair 1 2,  Robin R Hodges 1 2,  Tor P Utheim 1 2 3 4,  Charles N Serhan 6,  Darlene A Dartt 1 2
Affiliations

Affiliations

  • 1 Schepens Eye Research Institute/Massachusetts Eye and Ear, Boston, Massachusetts.
  • 2 Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts.
  • 3 Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
  • 4 Department of Plastic and Reconstructive Surgery, Oslo University Hospital, Oslo, Norway.
  • 5 Faculty of Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
  • 6 Department of Anesthesiology, Perioperative and Pain Medicine, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
PMID: 32510663 DOI: 10.1002/jcp.29846
Abstract

Mucin secretion from conjunctival goblet cells forms the tear film Mucin layer and requires regulation to function properly. Maresin 1 (MaR1) is a specialized proresolving mediator produced during the resolution of inflammation. We determined if MaR1 stimulates Mucin secretion and signaling pathways used. Cultured rat conjunctival goblet cells were used to measure the increase in intracellular CA2+ ([CA2+ ]i ) concentration and Mucin secretion. MaR1-increased [CA2+ ]i and secretion were blocked by inhibitors of Phospholipase C, protein kinase C, CA2+ /calmodulin-dependent protein kinase II, and extracellular-regulated kinase 1/2. MaR1 added before addition of histamine counterregulated histamine-stimulated increase in [CA2+ ]i and secretion. We conclude that MaR1 likely has two actions in conjunctival goblet cells: first, maintaining optimal tear film Mucin levels by increasing [CA2+ ]i and stimulating Mucin secretion in health and, second, attenuating the increase in [CA2+ ]i and overproduction of Mucin secretion by counterregulating the effect of histamine as occurs in ocular allergy.

Keywords

conjunctiva; goblet cell; signal transduction; specialized proresolving mediator (SPM).

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