2. Academic Validation
  3. Antimicrobial activity of harzianic acid against Staphylococcus pseudintermedius

Antimicrobial activity of harzianic acid against Staphylococcus pseudintermedius

  • Nat Prod Res. 2021 Dec;35(23):5440-5445. doi: 10.1080/14786419.2020.1779714.
Anna De Filippis 1 2,  Francesca Paola Nocera 1,  Simona Tafuri 1,  Francesca Ciani 1,  Alessia Staropoli 3 4,  Ernesto Comite 3,  Assunta Bottiglieri 3,  Laura Gioia 3,  Matteo Lorito 3 4,  Sheridan Lois Woo 3 4,  Francesco Vinale 1 4,  Luisa De Martino 1
Affiliations

Affiliations

  • 1 Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
  • 2 Department of Pharmacy, University of Naples Federico II, Naples, Italy.
  • 3 Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy.
  • 4 Institute for Sustainable Plant Protection, National Research Council, Portici, Italy.
PMID: 32538678 DOI: 10.1080/14786419.2020.1779714
Abstract

The emerging concern about the increase of Antibiotic resistance has encouraged research efforts to develop effective alternatives to counteract Bacterial infections. Herein, we studied a new perspective to therapeutic treatment against Staphylococcus pseudintermedius, an opportunistic pathogen documented as the major cause of skin, ear, and post-operative Bacterial infections in dogs and cats. Antimicrobial activity of secondary metabolites produced by selected microbial strains belonging to Trichoderma, Talaromyces, Clonostachys and Coniothyrium Fungal genera has been tested against S. pseudintermedius. Several extracts, particularly those obtained from Trichoderma harzianum E45 and ET45, showed a significant antimicrobial activity towards S. pseudintermedius methicillin-resistant (MRSP) and methicillin-susceptible (MSSP) strains. Bioassay-guided fractionation of E45 and ET45 extracts allowed to isolate harzianic acid as the major compound responsible for biological activities (e.g. antimicrobial, antibiofilm formation and biofilm disaggregation).

Keywords

Clonostachys; Coniothyrium; Staphylococcus pseudintermedius; Talaromyces; Trichoderma; antibiotic resistance; harzianic acid; secondary metabolites.

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