2. Academic Validation
  3. Broad assessment of bioactivity of a collection of spiroindane pyrrolidines through "cell painting"

Broad assessment of bioactivity of a collection of spiroindane pyrrolidines through "cell painting"

  • Bioorg Med Chem. 2020 Jul 1;28(13):115547. doi: 10.1016/j.bmc.2020.115547.
Manvendra Singh 1,  Nathan Garza 1,  Zachary Pearson 1,  Justin Douglas 2,  Zarko Boskovic 3
Affiliations

Affiliations

  • 1 Department of Medicinal Chemistry, University of Kansas, Lawrence, 66045 KS, United States.
  • 2 Department of Medicinal Chemistry, University of Kansas, Lawrence, 66045 KS, United States; Nuclear Magnetic Resonance Laboratory, University of Kansas, Lawrence, 66045 KS, United States.
  • 3 Department of Medicinal Chemistry, University of Kansas, Lawrence, 66045 KS, United States. Electronic address: zarko@ku.edu.
PMID: 32546297 DOI: 10.1016/j.bmc.2020.115547
Abstract

A collection of small molecules has been synthesized by composing photo-cycloaddition, C-H functionalization, and N-capping strategies. Multidimensional biological fingerprints of molecules comprising this collection have been recorded as changes in cell and organelle morphology. This untargeted, phenotypic approach allowed for a broad assessment of biological activity to be determined. Reproducibility and the magnitude of measured fingerprints revealed activity of several treatments. Reactive functional groups, such as imines, dominated the observed activity. Two non-reactive candidate compounds with distinct bioactivity fingerprints were identified, as well.

Keywords

Biological activity; Complexity; Fingerprints; Photochemistry.

Figures
Products