2. Academic Validation
  3. Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation

Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation

  • Sci Adv. 2020 Jul 22;6(30):eaba2987. doi: 10.1126/sciadv.aba2987.
Geng Dou 1,  Ran Tian 2,  Xuemei Liu 1 3,  Pingyun Yuan 2,  Qianwen Ye 1,  Jin Liu 1,  Siying Liu 1,  Jun Zhou 1,  Zhihong Deng 1,  Xin Chen 2,  Shiyu Liu 1,  Yan Jin 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
  • 2 Department of Chemical Engineering, Shaanxi Key Laboratory of Energy Chemical Process Intensification, Institute of Polymer Science in Chemical Engineering, School of Chemical Engineering and Technology, Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, China.
  • 3 Department of Paediatric Dentistry, School of Stomatology, China Medical University, Shenyang, Liaoning, 110002 China.
PMID: 32832662 DOI: 10.1126/sciadv.aba2987
Abstract

Engineered extracellular vesicles (EVs) carrying therapeutic molecules are promising candidates for disease therapies. Yet, engineering EVs with optimal functions is a challenge that requires careful selection of functionally specific vesicles and a proper engineering strategy. Here, we constructed chimeric apoptotic bodies (cABs) for on-demand inflammation modulation by combining pure membrane from apoptotic bodies (ABs) as a bioconjugation/regulation module and mesoporous silica nanoparticles (MSNs) as a carrier module. MSNs were preloaded with anti-inflammatory agents (microRNA-21 or curcumin) and modified with stimuli-responsive molecules to achieve accurate cargo release at designated locations. The resulting cABs actively target macrophages in the inflammatory region and effectively promote M2 polarization of these macrophages to modulate inflammation due to the synergistic regulatory effects of AB membranes and the intracellular release of preloaded cargos. This work provides strategies to arbitrarily engineer modular EVs that integrate the advantages of natural EVs and synthetic Materials for various applications.

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