2. Academic Validation
  3. Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1

Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1

  • J Agric Food Chem. 2020 Sep 30;68(39):10678-10684. doi: 10.1021/acs.jafc.0c03735.
Xia Zhang 1,  Min Gan 2,  Jingyun Li 3,  Hui Li 1,  Meicheng Su 1,  Dongfei Tan 1,  Shaolei Wang 1,  Man Jia 1,  Liguo Zhang 3,  Gang Chen 1
Affiliations

Affiliations

  • 1 Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • 2 Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • 3 Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
PMID: 32866000 DOI: 10.1021/acs.jafc.0c03735
Abstract

l-tryptophan (Trp) is widely used in food and feed enforcement to play an important role in biological processes. Various metabolites of Trp perform its potent function. The indole pyruvate pathway is one of the main pathways of Trp metabolism in the gut microbiota, providing numerous indole-derivatives, which can modulate intestinal homeostasis and mucosal immunity by activating the Aryl Hydrocarbon Receptor (AHR) signaling pathway. In this study, we constructed an IL4I1-overexpressed 293T cell line and found that IL4I1 can catalyze Trp to produce indole-3-acetic acid (IAA) and indole-3-carboxaldehyde (IAld). Moreover, both IAA and IAld are accumulated in dendritic cells (DCs) and can stimulate the expression of CYP1A1. Our results demonstrate the existence of the indole pyruvate pathway in host cells with IL4I1 as the key Enzyme. The IL4I1-mediated Trp metabolism implies the role of dietary impact on immunity.

Keywords

AHR signaling; IAA; IAld; IL4I1; indole pyruvate pathway.

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