2. Academic Validation
  3. Myricanol 5-fluorobenzyloxy ether regulation of survivin pathway inhibits human lung adenocarcinoma A549 cells growth in vitro

Myricanol 5-fluorobenzyloxy ether regulation of survivin pathway inhibits human lung adenocarcinoma A549 cells growth in vitro

  • BMC Complement Med Ther. 2020 Sep 3;20(1):269. doi: 10.1186/s12906-020-03062-8.
Guan-Hai Dai 1,  Xuan Chen 2,  Ze-Ming Ren 2,  Chen-Jie Dai 3,  Ye-Ling Tong 2,  Ke-Qun Chai 4
Affiliations

Affiliations

  • 1 Zhejiang Academy of Traditional Chinese Medicine, Institute of Basic Medicine, Hangzhou, 310007, China. daiguanhai@gmail.com.
  • 2 Zhejiang Academy of Traditional Chinese Medicine, Institute of Basic Medicine, Hangzhou, 310007, China.
  • 3 Zhejiang University-University of Edinburgh Institute, Zhejiang University, Haining, 314400, China.
  • 4 Oncology Department, Tongde Hospital of Zhejiang Province, Hangzhou, 310012, China. ckq_official@163.com.
PMID: 32883260 DOI: 10.1186/s12906-020-03062-8
Abstract

Background: This study aimed to explore the growth inhibitory effect of myricanol 5-fluorobenzyloxy ether (5FEM) and its underlying mechanisms in human lung adenocarcinoma A549 cells in vitro.

Methods: 5FEM was obtained by the chemical modification of myricanol with fluorobenzyloxy ether at the OH(5) position. The cytotoxicity, cell Apoptosis, cell cycle, mitochondrial membrane potential (ΔΨm), scratch test, colony formation, and the expression levels of the key Survivin pathway-related genes in A549 were evaluated.

Results: 5FEM could significantly inhibit A549 cell growth; induce cell apoptosis; increase G0/G1 population; reduce ΔΨm; inhibit cell migration and colony formation; upregulate caspase-9, P21, and Bax expression levels; and downregulate PARP, Survivin, and Bcl-2 expression level.

Conclusion: These results enhanced our understanding of 5FEM and aid the discovery of novel myricanol derivatives as potential antitumor agents.

Keywords

Antitumor; Cell growth inhibition; Human lung adenocarcinoma A549; Mitochondrial membrane potential; Myricanol 5-fluorobenzyloxy ether; Survivin pathway.

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