Cyanidin-3-O-Glucoside Improves Colonic Motility During Severe Acute Pancreatitis by Inhibiting the H2S-Regulated AMPK/mTOR Pathway

Background: Cyanidin-3-O-glucoside (C3G) is an important anthocyanin that can modulate digestive system functioning. Inflammation associated with severe acute pancreatitis (SAP) induces H₂S production, which impairs the gastrointestinal (GI) system. We investigated the effects of C3G in attenuating SAP-associated colonic motility loss by examining the H₂S level and activity of AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway.

Methods: A rat model of SAP was induced using sodium taurocholate, and the effect of C3G on colonic mobility, H₂S production, and the inflammatory response was investigated. AMPK/mTOR pathway changes were detected to assess the pathways by which H₂S influences colonic mobility in SAP-model rats. The mechanism underlying H₂S function was further examined by subjecting colonic muscle cells (CMCs) to C3G, SAP plasma and an AMPK Activator.

Results: Administering C3G improved colonic motility but suppressed the inflammatory response and H₂S production in the SAP-model rats, which was associated with inhibiting the AMPK/mTOR pathway. Furthermore, activating the AMPK/mTOR pathway in CMCs promoted inflammation but suppressed Ca2+ levels, even after administering C3G.

Conclusion: Administering C3G may improve SAP-associated colonic mobility by inhibiting the H₂S-mediated AMPK/mTOR pathway.