1. Academic Validation
  2. Retinoprotective Effect of Wild Olive (Acebuche) Oil-Enriched Diet against Ocular Oxidative Stress Induced by Arterial Hypertension

Retinoprotective Effect of Wild Olive (Acebuche) Oil-Enriched Diet against Ocular Oxidative Stress Induced by Arterial Hypertension

  • Antioxidants (Basel). 2020 Sep 18;9(9):885. doi: 10.3390/antiox9090885.
Álvaro Santana-Garrido 1 2 Claudia Reyes-Goya 1 M Carmen Pérez-Camino 3 Helder André 4 Alfonso Mate 1 2 Carmen M Vázquez 1 2
Affiliations

Affiliations

  • 1 Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, E-41012 Sevilla, Spain.
  • 2 Epidemiología Clínica y Riesgo Cardiovascular, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, E-41013 Sevilla, Spain.
  • 3 Departamento de Caracterización y Calidad de lípidos, Instituto de la Grasa-CSIC, E-41013 Sevilla, Spain.
  • 4 Department of Clinical Neuroscience, St. Erik Eye Hospital, Karolinska Institutet, 11282 Stockholm, Sweden.
Abstract

Oxidative stress plays an important role in the pathogenesis of ocular diseases, including hypertensive eye diseases. The beneficial effects of olive oil on cardiovascular diseases might rely on minor constituents. Currently, very little is known about the chemical composition and/or therapeutic effects of the cultivated olive tree's counterpart, wild olive (also known in Spain as acebuche-ACE). Here, we aimed to analyze the antioxidant and retinoprotective effects of ACE oil on the eye of hypertensive mice made hypertensive via administration of NG-nitro-L-arginine-methyl-ester (L-NAME), which were subjected to a dietary supplementation with either ACE oil or extra virgin olive oil (EVOO) for comparison purposes. Deep analyses of major and minor compounds present in both oils was accompanied by blood pressure monitoring, morphometric analyses, as well as different determinations of oxidative stress-related parameters in retinal layers. Aside from its antihypertensive effect, an ACE oil-enriched diet reduced NADPH (nicotinamide adenine dinucleotide phosphate) oxidase activity/gene/protein expression (with a major implication of NADPH Oxidase (NOX)2 isoform) in the retinas of hypertensive mice. Supplementation with ACE oil in hypertensive Animals also improved alterations in nitric oxide bioavailability and in antioxidant Enzyme profile. Interestingly, our findings show that the use of ACE oil resulted in better outcomes, compared with reference EVOO, against hypertension-related oxidative retinal damage.

Keywords

NADPH (nicotinamide adenine dinucleotide phosphate) oxidase; acebuche; arterial hypertension; nitric oxide; oxidative stress; retina; wild olive oil.

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