2. Academic Validation
  3. Natural iridoids from Patrinia heterophylla showing anti-inflammatory activities in vitro and in vivo

Natural iridoids from Patrinia heterophylla showing anti-inflammatory activities in vitro and in vivo

  • Bioorg Chem. 2020 Nov;104:104331. doi: 10.1016/j.bioorg.2020.104331.
Peng Wu 1,  Ziteng Song 1,  Ying Li 1,  Huimei Wang 1,  Han Zhang 1,  Jiahe Bao 1,  Yuhao Li 2,  Jianlin Cui 2,  Da-Qing Jin 2,  Aijie Wang 3,  Bin Liang 4,  Dongho Lee 5,  Jing Xu 6,  Yuanqiang Guo 7
Affiliations

Affiliations

  • 1 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300050, People's Republic of China.
  • 2 School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.
  • 3 Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China; School of Civil & Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, People's Republic of China. Electronic address: aijiepublication@126.com.
  • 4 School of Civil & Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, People's Republic of China.
  • 5 Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
  • 6 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300050, People's Republic of China. Electronic address: xujing611@nankai.edu.cn.
  • 7 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300050, People's Republic of China. Electronic address: victgyq@nankai.edu.cn.
PMID: 33142407 DOI: 10.1016/j.bioorg.2020.104331
Abstract

Inflammation, especially chronic inflammation, has been found to be closely related to the pathology of many diseases and the discovery of bioactive Natural Products to inhibit NO production is one of strategies to treat inflammation. In our continuous search for bioactive natural substances as potential anti-inflammatory agents, five new compounds (1-5) were extracted and purified from Patrinia heterophylla. The NMR and MS data analysis, along with electronic circular dichroism (ECD) calculations, led to the identification of these isolates, which were new Iridoids. Using cell and zebrafish models, the in vitro and in vivo anti-inflammatory effects were conducted to evaluate the potency of anti-inflammation of these compounds. The preliminary mechanism was explored using molecular docking and Western blotting experiments.

Keywords

Anti-inflammatory potency; Iridoids; NO inhibition; Patrinia heterophylla; Zebrafish model.

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