1. Academic Validation
  2. Tim-3 deteriorates neuroinflammatory and neurocyte apoptosis after subarachnoid hemorrhage through the Nrf2/HMGB1 signaling pathway in rats

Tim-3 deteriorates neuroinflammatory and neurocyte apoptosis after subarachnoid hemorrhage through the Nrf2/HMGB1 signaling pathway in rats

  • Aging (Albany NY). 2020 Nov 7;12(21):21161-21185. doi: 10.18632/aging.103796.
Shenquan Guo 1 Yuanzhi Li 1 2 Boyang Wei 1 Wenchao Liu 1 Ran Li 1 Wenping Cheng 1 Xin Zhang 1 Xuying He 1 Xifeng Li 1 Chuanzhi Duan 1
Affiliations

Affiliations

  • 1 The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • 2 Department of Neurosurgery, Affiliated Hengyang Hospital, Southern Medical University (Hengyang Central Hospital), Hengyang, China.
Abstract

Inflammation is known to play an important role in early brain injury (EBI) after subarachnoid hemorrhage (SAH). T cell immunoglobulin and Mucin domain-3 (TIM-3) has emerged as a critical regulator of adaptive and innate immune responses, and has been identified to play a vital role in certain inflammatory diseases; The present study explored the effect of TIM-3 on inflammatory responses and detailed mechanism in EBI following SAH. We investigated the effects of TIM-3 on SAH models established by endovascular puncture method in Sprague-Dawley rats. The present studies revealed that SAH induced a significant inflammatory response and significantly increased TIM-3 expression. Tim-3-AAV administration aggravated neurocyte Apoptosis, brain edema, blood-brain barrier permeability, and neurological dysfunction; significantly inhibited Nrf2 expression; and increased HMGB1 expression and secretion of pro-inflammatory cytokines, such as tumor necrosis factor alpha, interleukin (IL)-1 beta, IL-17, and IL-18. However, TIM-3 siRNA or NK252 administration abolished the pro-inflammatory effects of TIM-3. Our results indicate a function for TIM-3 as a molecular player that links neuroinflammation and brain damage after SAH. We reveal that TIM-3 overexpression deteriorates neuroinflammatory and neurocyte Apoptosis after subarachnoid hemorrhage through the Nrf2/HMGB1 signaling pathway in rats.

Keywords

HMGB1; Tim-3; early brain injury; neuroinflammation; subarachnoid hemorrhage.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-19734
    99.48%, Nrf2 Activator