2. Academic Validation
  3. Immunofluorescence Staining of Paraffin Sections Step by Step

Immunofluorescence Staining of Paraffin Sections Step by Step

  • Front Neuroanat. 2020 Nov 9:14:582218. doi: 10.3389/fnana.2020.582218.
Sami Zaqout 1,  Lena-Luise Becker 2 3 4,  Angela M Kaindl 2 3 4
Affiliations

Affiliations

  • 1 Basic Medical Science Department, College of Medicine, QU Health, Qatar University, Doha, Qatar.
  • 2 Charité - Universitätsmedizin Berlin, Institute of Cell and Neurobiology, Berlin, Germany.
  • 3 Charité - Universitätsmedizin Berlin, Center for Chronically Sick Children (Sozialpädiatrisches Zentrum, SPZ), Berlin, Germany.
  • 4 Charité - Universitätsmedizin Berlin, Department of Pediatric Neurology, Berlin, Germany.
PMID: 33240048 DOI: 10.3389/fnana.2020.582218
Abstract

Immunofluorescence staining is the most frequently applied technique to detect and visualize various molecules in biological samples. Many protocols can be found in the literature and the websites of commercial antibody producers. This can result in a time-consuming and costly methodical work to establish "simple" antibody staining. We here summarize in a stepwise manner an easy-to-follow immunofluorescence staining protocol with an improved specific fluorescent signal and a reduced background and non-specific binding signal. This will help scientists to save time, effort, and antibody costs during the application of such a valuable technique.

Keywords

antibodies; imaging; immunofluorescence; microscopy; paraffin sections.

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