2. Academic Validation
  3. Glycolysis Combined with Core Pluripotency Factors to Promote the Formation of Chicken Induced Pluripotent Stem Cells

Glycolysis Combined with Core Pluripotency Factors to Promote the Formation of Chicken Induced Pluripotent Stem Cells

  • Animals (Basel). 2021 Feb 6;11(2):425. doi: 10.3390/ani11020425.
Xia Yuan 1 2,  Chen Zhang 1 2,  Ruifeng Zhao 1 2,  Jingyi Jiang 1 2,  Xiang Shi 1 2,  Ming Zhang 1 2,  Hongyan Sun 1 2,  Qisheng Zuo 1 2,  Yani Zhang 1 2,  Jiuzhou Song 3,  Guohong Chen 1 2,  Bichun Li 1 2
Affiliations

Affiliations

  • 1 Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
  • 2 Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
  • 3 Department of Animal & Avian Sciences, University of Maryland, College Park, MD 20742, USA.
PMID: 33562170 DOI: 10.3390/ani11020425
Abstract

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) in vitro. Previously, a lentivirus induction strategy of introducing Oct4, Sox2, Nanog and Lin28 (OSNL) into the iPSC process has been shown as a possible way to produce chicken iPSCs from chicken embryonic fibroblasts, but the induction efficiency of this method was found to be significantly limiting. In order to help resolve this efficiency obstacle, this study seeks to clarify the associated regulation mechanisms and optimizes the reprogramming strategy of chicken iPSCs. This study showed that glycolysis and the expression of glycolysis-related genes correlate with a more efficient reprogramming process. At the same time, the transcription factors Oct4, Sox2 and Nanog were found to activate the expression of glycolysis-related genes. In addition, we introduced two small-molecule inhibitors (2i-SP) as a "glycolysis activator" together with the OSNL cocktail, and found that this significantly improved the induction efficiency of the iPSC process. As such, the study identifies direct molecular connections between core pluripotency factors and glycolysis during the chicken iPSC induction process and, with its results, provides a theoretical basis and technical support for chicken somatic reprogramming.

Keywords

chicken iPSCs; core pluripotency factors; glycolysis; reprogramming.

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