2. Academic Validation
  3. Epo-C12 inhibits peroxiredoxin 1 peroxidase activity

Epo-C12 inhibits peroxiredoxin 1 peroxidase activity

  • Bioorg Med Chem. 2021 Jul 1:41:116203. doi: 10.1016/j.bmc.2021.116203.
Tomoka Yoda 1,  Masateru Furuta 1,  Tomohiko Tsutsumi 1,  Seiki Ikeda 1,  Shunsuke Yukizawa 1,  Satoshi Arai 1,  Akinori Morita 2,  Kenji Yamatoya 3,  Kazuya Nakata 4,  Shusuke Tomoshige 5,  Kenji Ohgane 1,  Yuuki Furuyama 1,  Kengo Sakaguchi 1,  Fumio Sugawara 1,  Susumu Kobayashi 6,  Masahiko Ikekita 1,  Kouji Kuramochi 7
Affiliations

Affiliations

  • 1 Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
  • 2 Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Department of Biomedical Science and Technology, Graduate School of Biomedical Sciences, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
  • 3 Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu-City, Chiba, 279-0021, Japan.
  • 4 Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo, 184-0012, Japan.
  • 5 Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
  • 6 Department of Pharmacy, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
  • 7 Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. Electronic address: kuramoch@rs.tus.ac.jp.
PMID: 34015702 DOI: 10.1016/j.bmc.2021.116203
Abstract

Epo-C12 is a synthetic derivative of epolactaene, isolated from Penicillium sp. BM 1689-P. Epo-C12 induces Apoptosis in human acute lymphoblastoid leukemia BALL-1 cells. In our previous studies, seven proteins that bind to Epo-C12 were identified by a combination of pull-down experiments using biotinylated Epo-C12 (Bio-Epo-C12) and mass spectrometry. In the present study, the effect of Epo-C12 on peroxiredoxin 1 (Prx 1), one of the proteins that binds to Epo-C12, was investigated. Epo-C12 inhibited Prx 1 peroxidase activity. However, it did not suppress its chaperone activity. Binding experiments between Bio-Epo-C12 and point-mutated Prx 1s suggest that Epo-C12 binds to Cys52 and Cys83 in Prx 1. The present study revealed that Prx 1 is one of the target proteins through which Epo-C12 exerts an apoptotic effect in BALL-1 cells.

Keywords

Chaperone; Epolactaene; Peroxidase; Peroxiredoxin 1; Reactive oxygen species.

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