2. Academic Validation
  3. Agrimol B present in Agrimonia pilosa Ledeb impedes cell cycle progression of cancer cells through G0 state arrest

Agrimol B present in Agrimonia pilosa Ledeb impedes cell cycle progression of cancer cells through G0 state arrest

  • Biomed Pharmacother. 2021 Sep;141:111795. doi: 10.1016/j.biopha.2021.111795.
Su Su Thae Hnit 1,  Rongzhen Ding 2,  Ling Bi 2,  Chanlu Xie 1,  Mu Yao 1,  Paul De Souza 3,  Ling Xu 2,  Zhong Li 4,  Qihan Dong 5
Affiliations

Affiliations

  • 1 Chinese Medicine Anti-Cancer Evaluation Program, Greg Brown Laboratory, Central Clinical School and Charles Perkins Centre, The University of Sydney, Sydney, Australia; Department of Endocrisnology, Royal Prince Alfred Hospital, Sydney, Australia.
  • 2 Department of Oncology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  • 3 School of Medicine, Western Sydney University, Australia.
  • 4 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. Electronic address: lizhong@bucm.edu.cn.
  • 5 Chinese Medicine Anti-Cancer Evaluation Program, Greg Brown Laboratory, Central Clinical School and Charles Perkins Centre, The University of Sydney, Sydney, Australia; Department of Endocrisnology, Royal Prince Alfred Hospital, Sydney, Australia. Electronic address: Qihan.Dong@sydney.edu.au.
PMID: 34098217 DOI: 10.1016/j.biopha.2021.111795
Abstract

Cancer recurrence poses a significant challenge. At the cellular level, recurrence takes place as a result of reactivation of dormant Cancer cells residing at G0 phase. The aim of the study was to identify compounds that can trap prostate and lung Cancer cells in G0 phase from a new Chinese herb recipe, Astringent recipe, consisting of Radix Paeoniae Alba, Agrimonia pilosa Ledeb, Fructus Mume, Fritillaria thunbergii Miq., Ganoderma Lucidum Karst, and Astragalus membranaceus (Fisch.) Bunge. Astringent recipe impeded cell cycle progression in prostate and lung Cancer cells by rounding them up at G0 phase by flow cytometric analysis of Cancer cells stained with Hoechst 33342 and Pyronin Y, respectively, for DNA and RNA. The anti-cancer efficacy of the recipe was found to be attributable to Agrimonia pilosa Ledeb. Further study established that agrimol B, a polyphenol derived from Agrimonia pilosa Ledeb, contributed to the activity of the herb. The action of agrimol B on the Cancer cells was likely derived from its effect on c-Myc, SKP2 and p27 by immunoblotting and immunofluorescence. Oral administration of Agrimonia pilosa Ledeb or agrimol B reduced growth of prostate Cancer cell xenograft in animal. In conclusion, Agrimol B can enrich for prostate and lung Cancer cells in G0 state and influence key regulators that govern G0 status.

Keywords

Agrimol B; Ellagic acid; Lung cancer; Polyphenols; Prostate cancer; Quiescent cancer cells.

Figures
Products