1. Academic Validation
  2. Hydrogen sulfide stimulates lipid biogenesis from glutamine that is dependent on the mitochondrial NAD(P)H pool

Hydrogen sulfide stimulates lipid biogenesis from glutamine that is dependent on the mitochondrial NAD(P)H pool

  • J Biol Chem. 2021 Aug;297(2):100950. doi: 10.1016/j.jbc.2021.100950.
Sebastian Carballal 1 Victor Vitvitsky 2 Roshan Kumar 2 David A Hanna 2 Marouane Libiad 2 Aditi Gupta 2 Jace W Jones 3 Ruma Banerjee 4
Affiliations

Affiliations

  • 1 Department of Biological Chemistry, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA; Departamento de Bioquímica, Facultad de Medicina and Centro de Investigaciones Biomédicas (CEINBIO), Universidad de la República, Montevideo, Uruguay.
  • 2 Department of Biological Chemistry, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA.
  • 3 Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, Maryland, USA.
  • 4 Department of Biological Chemistry, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA. Electronic address: rbanerje@umich.edu.
Abstract

Mammalian cells synthesize H2S from sulfur-containing Amino acids and are also exposed to exogenous sources of this signaling molecule, notably from gut microbes. As an inhibitor of complex IV in the electron transport chain, H2S can have a profound impact on metabolism, suggesting the hypothesis that metabolic reprogramming is a primary mechanism by which H2S signals. In this study, we report that H2S increases lipogenesis in many cell types, using carbon derived from glutamine rather than from glucose. H2S-stimulated lipid synthesis is sensitive to the mitochondrial NAD(P)H pools and is enabled by reductive carboxylation of α-ketoglutarate. Lipidomics analysis revealed that H2S elicits time-dependent changes across several lipid classes, e.g., upregulating triglycerides while downregulating phosphatidylcholine. Direct analysis of triglyceride concentration revealed that H2S induces a net increase in the size of this lipid pool. These results provide a mechanistic framework for understanding the effects of H2S on increasing lipid droplets in adipocytes and population studies that have pointed to a positive correlation between cysteine (a substrate for H2S synthesis) and fat mass.

Keywords

NAD(P)H; bioenergetics; cell metabolism; electron transport chain; hydrogen sulfide; lipid synthesis; lipidomic; metabolic reprogramming; redox signaling; reductive carboxylation.

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