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  3. Comparison of gene expression and activation of transcription factors in organoid-derived monolayer intestinal epithelial cells and organoids

Comparison of gene expression and activation of transcription factors in organoid-derived monolayer intestinal epithelial cells and organoids

  • Biosci Biotechnol Biochem. 2021 Sep 22;85(10):2137-2144. doi: 10.1093/bbb/zbab136.
Yu Takahashi 1,  Yu Inoue 1,  Keitaro Kuze 1,  Shintaro Sato 2,  Makoto Shimizu 3,  Hiroshi Kiyono 4,  Yoshio Yamauchi 1,  Ryuichiro Sato 1 3 5
Affiliations

Affiliations

  • 1 Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
  • 2 Mucosal Vaccine Project, BIKEN Innovative Vaccine Research Alliance Laboratories, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • 3 Nutri-Life Science Laboratory, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.
  • 4 Division of Innate Immune Regulation, International Research and Development Center for Mucosal Vaccine, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
  • 5 AMED-CREST, Japan Agency for Medical Research and Development, Tokyo, Japan.
PMID: 34297057 DOI: 10.1093/bbb/zbab136
Abstract

Intestinal organoids better represent in vivo intestinal properties than conventionally used established cell lines in vitro. However, they are maintained in three-dimensional culture conditions that may be accompanied by handling complexities. We characterized the properties of human organoid-derived two-dimensionally cultured intestinal epithelial cells (IECs) compared with those of their parental organoids. We found that the expression of several intestinal markers and functional genes were indistinguishable between monolayer IECs and organoids. We further confirmed that their specific ligands equally activate intestinal ligand-activated transcriptional regulators in a dose-dependent manner. The results suggest that culture conditions do not significantly influence the fundamental properties of monolayer IECs originating from organoids, at least from the perspective of gene expression regulation. This will enable their use as novel biological tools to investigate the physiological functions of the human intestine.

Keywords

induced pluripotent stem cells; intestinal organoids; monolayer intestinal epithelial cells.

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