2. Academic Validation
  3. Diazinon toxicity in hepatic and spleen mononuclear cells is associated to early induction of oxidative stress

Diazinon toxicity in hepatic and spleen mononuclear cells is associated to early induction of oxidative stress

  • Int J Environ Health Res. 2022 Oct;32(10):2309-2323. doi: 10.1080/09603123.2021.1962814.
Manuel Iván Girón-Pérez 1,  Verónica S Mary 2 3,  Héctor R Rubinstein 2 3,  Gladys A Toledo-Ibarra 1,  Martín G Theumer 2 3
Affiliations

Affiliations

  • 1 Universidad Autónoma de Nayarit,Secretaría de Investigación Y Posgrado, Laboratorio de Inmunotoxicología, Boulevard Tepic-Xalisco S/n, Cd de La Cultura Amado Nervo, C.P, Tepic, Nayarit, Mexico.
  • 2 Departamento De Bioquímica Clínica, Facultad De Ciencias Químicas, Universidad Nacional De Córdoba (UNC), Córdoba, Argentina.
  • 3 Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Centro de Investigaciones En Bioquímica Clínica E Inmunología (CIBICI), Córdoba, Argentina.
PMID: 34404283 DOI: 10.1080/09603123.2021.1962814
Abstract

Diazinon is an organophosphorus pesticide, which may have potential toxic effects on the liver and immune system; however, the underlying mechanisms remain mostly unidentified. This work is aimed at evaluating the oxidative stress and cell cycle alterations elicited by low-dose diazinon in a rat liver cell line (BRL-3A) and spleen mononuclear cells (SMC) from Wistar rats. Diazinon (10-50 μM) caused early Reactive Oxygen Species (ROS) generation (from 4 h) as well as increased O2•- level (from 0.5 h), which led to subsequent lipid peroxidation at 24 h, in BRL-3A cells. In SMC, diazinon (20 μM) produced similar increases in ROS levels, at 4 and 24 h, with the highest O2•- level being found at 4 h. Low-dose diazinon induced G1-phase arrest and cell death in hepatic cells and SMC. Therefore, diazinon could affect the liver and the immunological system through the premature oxidative stress induction.Abbreviations: O2•-: superoxide anion radical; ROS: reactive oxygen species; SMC: spleen mononuclear cells; TBARS: thiobarbituric acid reactive substances.

Keywords

Diazinon; Hepatotoxicity; ROS; immunotoxicity.

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