2. Academic Validation
  3. Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway

Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway

  • Lab Invest. 2022 Jan;102(1):90-101. doi: 10.1038/s41374-021-00675-6.
Kailun Zhang  # 1,  Bo Chai  # 2,  Hao Ji  # 1,  Liuqing Chen 1,  Yanbing Ma 1,  Lifei Zhu 2,  Jingyu Xu 1,  Yanqing Wu 1,  Yinan Lan 3,  Hao Li 4,  Zhiguo Feng 2,  Jian Xiao 5,  Hongyu Zhang 6,  Ke Xu 7
Affiliations

Affiliations

  • 1 Institute of Life Sciences, Engineering Laboratory of Zhejiang province for pharmaceutical development of growth factors, Biomedical Collaborative Innovation Center of Wenzhou, Wenzhou University, Zhejiang, China.
  • 2 School of Pharmaceutical Sciences, Wenzhou Wound Repair and Regeneration Key Laboratory, Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, China.
  • 3 Department of Orthopedic Surgery, Lishui Central Hospital, The Fifth Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.
  • 4 Department of Orthopedics Surgery, Lishui People's Hospital, The sixth affiliated hospital of Wenzhou medical university, Lishui, Zhejiang, China.
  • 5 School of Pharmaceutical Sciences, Wenzhou Wound Repair and Regeneration Key Laboratory, Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, China. xfxj2000@126.com.
  • 6 School of Pharmaceutical Sciences, Wenzhou Wound Repair and Regeneration Key Laboratory, Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, China. st_hyz@126.com.
  • 7 Institute of Life Sciences, Engineering Laboratory of Zhejiang province for pharmaceutical development of growth factors, Biomedical Collaborative Innovation Center of Wenzhou, Wenzhou University, Zhejiang, China. godxu1987@163.com.
  • # Contributed equally.
PMID: 34521991 DOI: 10.1038/s41374-021-00675-6
Abstract

Bioactive glass (BG) has recently shown great promise in soft tissue repair, especially in wound healing; however, the underlying mechanism remains unclear. Pyroptosis is a novel type of programmed cell death that is involved in various traumatic injury diseases. Here, we hypothesized that BG may promote wound healing through suppression of Pyroptosis. To test this scenario, we investigated the possible effect of BG on Pyroptosis in wound healing both in vivo and in vitro. This study showed that BG can accelerate wound closure, granulation formation, collagen deposition, and angiogenesis. Moreover, western blot analysis and immunofluorescence staining revealed that BG inhibited the expression of pyroptosis-related proteins in vivo and in vitro. In addition, while BG regulated the expression of connexin43 (Cx43), it inhibited Reactive Oxygen Species (ROS) production. Cx43 activation and inhibition experiments further indicate that BG inhibited Pyroptosis in endothelial cells by decreasing Cx43 expression and ROS levels. Taken together, these studies suggest that BG promotes wound healing by inhibiting Pyroptosis via Cx43/ROS signaling pathway.

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