2. Academic Validation
  3. CHPF promotes gastric cancer tumorigenesis through the activation of E2F1

CHPF promotes gastric cancer tumorigenesis through the activation of E2F1

  • Cell Death Dis. 2021 Sep 25;12(10):876. doi: 10.1038/s41419-021-04148-y.
Xiaolin Lin  # 1,  Ting Han  # 1,  Qing Xia  # 1,  Jiujie Cui 1,  Meng Zhuo 1,  Yiyi Liang 1,  Wenyu Su 2,  Lisha Wang 3,  Liwei Wang 4,  Zebing Liu 5,  Xiuying Xiao 6
Affiliations

Affiliations

  • 1 Department of Oncology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.
  • 2 Department of Gastroenterology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Disease, Shanghai, 200127, China.
  • 3 Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, 48109, MI, USA.
  • 4 Department of Oncology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China. liweiwang@shsmu.edu.cn.
  • 5 Department of Pathology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China. zebing1311@yahoo.com.
  • 6 Department of Oncology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China. xiaoxiuying2002@163.com.
  • # Contributed equally.
PMID: 34564711 DOI: 10.1038/s41419-021-04148-y
Abstract

Chondroitin polymerizing factor (CHPF) is an important Glycosyltransferase involved in the biosynthesis of chondroitin sulfate. However, the relationship between CHPF and gastric Cancer has not been fully investigated. CHPF expression in gastric Cancer tissues was detected by immunohistochemistry and correlated with gastric Cancer patient prognosis. Cultured gastric Cancer cells and human gastric epithelial cell line GES1 were used to investigate the effects of shCHPF and shE2F1 on the development and progression of gastric Cancer by MTT, western blotting, flow cytometry analysis of cell Apoptosis, colony formation, transwell and gastric Cancer xenograft mouse models, in vitro and in vivo. In gastric Cancer tissues, CHPF was found to be significantly upregulated, and its expression correlated with tumor infiltration and advanced tumor stage and shorter patient survival in gastric Cancer. CHPF may promote gastric Cancer development by regulating cell proliferation, colony formation, cell Apoptosis and cell migration, while knockdown induced the opposite effects. Moreover, the results from in vivo experiments demonstrated that tumor growth was suppressed by CHPF knockdown. Additionally, E2F1 was identified as a potential downstream target of CHPF in the regulation of gastric Cancer, and its knockdown decreased the CHPF-induced promotion of gastric Cancer. Mechanistic study revealed that CHPF may regulate E2F1 through affecting UBE2T-mediated E2F1 ubiquitination. This study showed, for the first time, that CHPF is a potential prognostic indicator and tumor promoter in gastric Cancer whose function is likely carried out through the regulation of E2F1.

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