2. Academic Validation
  3. 4-Hydroxyderricin Promotes Apoptosis and Cell Cycle Arrest through Regulating PI3K/AKT/mTOR Pathway in Hepatocellular Cells

4-Hydroxyderricin Promotes Apoptosis and Cell Cycle Arrest through Regulating PI3K/AKT/mTOR Pathway in Hepatocellular Cells

  • Foods. 2021 Aug 29;10(9):2036. doi: 10.3390/foods10092036.
Xiang Gao 1,  Yuhuan Jiang 1,  Qi Xu 1,  Feng Liu 2 3,  Xuening Pang 1,  Mingji Wang 3,  Qun Li 3 4,  Zichao Li 1 3
Affiliations

Affiliations

  • 1 Institute of Biomedical Engineering, College of Life Sciences, Qingdao University, Qingdao 266071, China.
  • 2 Department of Horticultural Technology, Ningbo City College of Vocational Technology, Ningbo 315199, China.
  • 3 Joint Institute of Angelica keiskei Health Industry Technology, Qingdao University, Qingdao 266071, China.
  • 4 College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
PMID: 34574146 DOI: 10.3390/foods10092036
Abstract

4-hydroxyderricin (4-HD), as a natural flavonoid compound derived from Angelica keiskei, has largely unknown inhibition and mechanisms on liver Cancer. Herein, we investigated the inhibitory effects of 4-HD on hepatocellular carcinoma (HCC) cells and clarified the potential mechanisms by exploring Apoptosis and cell cycle arrest mediated via the PI3K/Akt/mTOR signaling pathway. Our results show that 4-HD treatment dramatically decreased the survival rate and activities of HepG2 and Huh7 cells. The protein expressions of apoptosis-related genes significantly increased, while those related to the cell cycle were decreased by 4-HD. 4-HD also down-regulated PI3K, p-PI3K, p-AKT, and p-mTOR protein expression. Moreover, PI3K Inhibitor (LY294002) enhanced the promoting effect of 4-HD on Apoptosis and cell cycle arrest in HCC cells. Consequently, we demonstrate that 4-HD can suppress the proliferation of HCC cells by promoting the PI3K/Akt/mTOR signaling pathway mediated Apoptosis and cell cycle arrest.

Keywords

Angelica keiskei; anti-tumor; apoptosis; cell cycle; chalcone; mechanism.

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