2. Academic Validation
  3. Cyclic helix B peptide promotes random-pattern skin flap survival via TFE3-mediated enhancement of autophagy and reduction of ROS levels

Cyclic helix B peptide promotes random-pattern skin flap survival via TFE3-mediated enhancement of autophagy and reduction of ROS levels

  • Br J Pharmacol. 2022 Jan;179(2):301-321. doi: 10.1111/bph.15702.
Junsheng Lou 1 2 3,  Haojie Zhang 1 2 3,  Jianjun Qi 4,  Yu Xu 1 2 3,  Xingyu Wang 1 2 3,  Jingtao Jiang 1 2 3,  Xinli Hu 1 2 3,  Libin Ni 1 2 3,  Yuepiao Cai 5,  Xiangyang Wang 1 2 3,  Weiyang Gao 1 2 3,  Jian Xiao 5,  Kailiang Zhou 1 2 3
Affiliations

Affiliations

  • 1 Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
  • 2 Zhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, China.
  • 3 The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
  • 4 Center of Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.
  • 5 Molecular Pharmacology Research Center, School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, China.
PMID: 34622942 DOI: 10.1111/bph.15702
Abstract

Background and purpose: Necrosis of random-pattern skin flaps limits their clinical application. Helix B surface peptide (HBSP) protects tissues from ischaemia-reperfusion injury but its short plasma half-life limits its applications. Here, we have synthesized cyclic helix B peptide (CHBP) and investigated its role in FLAP survival and the underlying mechanisms.

Experimental approach: FLAP viability was evaluated by survival area analysis, laser Doppler blood flow and histological analysis. RNA Sequencing was used to identify mechanisms underlying the effects of CHBP. Levels of Autophagy, oxidative stress, Pyroptosis, Necroptosis and molecules related to the AMP-activated protein kinase (AMPK)-TRPML1-calcineurin signalling pathway were assayed with Western blotting, RT-qPCR, immunohistochemistry and immunofluorescence.

Key results: The results indicated that CHBP promoted the survival of random-pattern skin flaps. The results of RNA Sequencing analysis indicated that Autophagy, oxidative stress, Pyroptosis and Necroptosis were involved in the ability of CHBP to promote skin FLAP survival. Restoration of Autophagy flux and enhanced resistance to oxidative stress contributed to inhibition of Pyroptosis and Necroptosis. Increased Autophagy and inhibition of oxidative stress in the ischaemic flaps were regulated by transcription factor E3 (TFE3). A decrease in the levels of TFE3 caused a reduction in Autophagy flux and accumulation of ROS and eliminated the protective effect of CHBP. Moreover, CHBP regulated the activity of TFE3 via the AMPK-TRPML1-calcineurin signalling pathway.

Conclusion and implications: CHBP promotes skin FLAP survival by up-regulating Autophagy and inhibiting oxidative stress in the ischaemic FLAP and may have potential clinical applications.

Keywords

CHBP; TFE3; autophagy; oxidative stress; random skin flaps.

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