2. Academic Validation
  3. Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system

Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system

  • STAR Protoc. 2021 Nov 5;2(4):100881. doi: 10.1016/j.xpro.2021.100881.
Mei Xu 1,  Wenhao Zhang 1,  Mengyang Geng 1,  Yiding Zhao 1,  Shengyi Sun 1,  Qian Gao 1,  Yan Liu 2,  Ling Shuai 1 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China.
  • 2 Department of Obstetrics, Tianjin First Central Hospital, Nankai University, Tianjin 300192, China.
  • 3 National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.
PMID: 34806042 DOI: 10.1016/j.xpro.2021.100881
Abstract

Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-term and can produce genome-wide mutants combined with transposons. It is promising in high-throughput genetic screening of trophoblast-specific modulators. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019).

Keywords

Cell Biology; Cell Differentiation; Developmental biology; Genetics; High Throughput Screening; Stem Cells.

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