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  3. The study of biological activity of mandelic acid and its alkali metal salts in wastewaters

The study of biological activity of mandelic acid and its alkali metal salts in wastewaters

  • Environ Res. 2022 Apr 1:205:112429. doi: 10.1016/j.envres.2021.112429.
Marzena Matejczyk 1,  Piotr Ofman 2,  Renata Świsłocka 3,  Monika Parcheta 3,  Włodzimierz Lewandowski 3
Affiliations

Affiliations

  • 1 Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Department of Chemistry, Biology and Biotechnology, Wiejska 45E Street, 15-341, Bialystok, Poland. Electronic address: m.matejczyk@pb.edu.pl.
  • 2 Bialystok University of Technology, Department of Environmental Engineering Technology, Bialystok University of Technology, Bialystok, 15-341, Poland.
  • 3 Bialystok University of Technology, Faculty of Civil Engineering and Environmental Sciences, Department of Chemistry, Biology and Biotechnology, Wiejska 45E Street, 15-341, Bialystok, Poland.
PMID: 34863693 DOI: 10.1016/j.envres.2021.112429
Abstract

In the present work we compared the biological activity of mandelic acid (MA) and its Li, Na, K, Rb and Cs salts. The study also investigated the effect of raw wastewaters (RW) and treated wastewaters (TW), comparable to microbial medium (MM) on the biological activity of the tested chemical compounds used in concentrations of 5; 2.5; 1.25; 0.625; 0.3125 mg/ml. In the present experiment the evaluation of the following parameters was performed: E. coli (ATCC 25922) cells viability, growth inhibition of E. coli (ATCC 25922), the inhibition of GFP protein, genotoxicity and ROS generation. Our results showed that three main factors differentiated the Antibacterial activity of MA and its Li, Na, K, Rb and Cs salts: study environment (MM, RW, TW), metal forming salt of mandelic acid and concentration of tested compounds. Additionally, raw and treated wastewater, compared to microbial medium, changes the antimicrobial activity of MA and its salts in relation to the E. coli strain. We also detected that both MA and its salts affect the GFP protein and the induction of the recA promoter (genotoxicity test). The activity of the tested salts in relation to these two parameters is strictly dependent on the type of salt-forming metal and the concentration used. The analysis of ROS synthesis suggests that in the majority of the studied mandelic acid salts, oxidative stress is the dominant mechanism of cytotoxicity and genotoxicity. We also showed that both raw wastewaters (RW) and treated wastewaters (TW), compared to microbial medium (MM), change significantly the activity of MA and its salts.

Keywords

Biological activity; Cs salts; K; Li; Mandelic acid (MA); Microbial biosensors; Na; Rb; Wastewaters.

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