2. Academic Validation
  3. Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains

Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains

  • Bioresour Technol. 2022 Feb;346:126638. doi: 10.1016/j.biortech.2021.126638.
Helena Gómez-Álvarez 1,  Pablo Iturbe 2,  Virginia Rivero-Buceta 1,  Paul Mines 3,  Timothy D H Bugg 4,  Juan Nogales 5,  Eduardo Díaz 6
Affiliations

Affiliations

  • 1 Margarita Salas Center for Biological Research, Spanish National Research Council, Ramiro de Maeztu 9, 28040 Madrid, Spain.
  • 2 Margarita Salas Center for Biological Research, Spanish National Research Council, Ramiro de Maeztu 9, 28040 Madrid, Spain; Navarrabiomed, University of Navarra, Irunlarrea 3, 31008 Pamplona, Spain.
  • 3 Biome Bioplastics Ltd, North Road, Marchwood, Southampton SO40 4BL, UK.
  • 4 Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom.
  • 5 National Centre for Biotechnology, Spanish National Research Council, Darwin 3, 28049 Madrid, Spain; Interdisciplinary Platform for Sustainable Plastics towards a Circular Economy-Spanish National Research Council (SusPlast-CSIC), Madrid, Spain.
  • 6 Margarita Salas Center for Biological Research, Spanish National Research Council, Ramiro de Maeztu 9, 28040 Madrid, Spain; Interdisciplinary Platform for Sustainable Plastics towards a Circular Economy-Spanish National Research Council (SusPlast-CSIC), Madrid, Spain. Electronic address: ediaz@cib.csic.es.
PMID: 34971782 DOI: 10.1016/j.biortech.2021.126638
Abstract

2,4 pyridine dicarboxylic acid (2,4 PDCA) is an analogue of terephthalate, and hence a target chemical in the field of bio-based plastics. Here, Pseudomonas putida KT2440 strains were engineered to efficiently drive the metabolism of lignin-derived monoaromatics towards 2,4 PDCA in a resting cells-based bioprocess that alleviates growth-coupled limitations and allows biocatalysts recycling. Native β-ketoadipate pathway was blocked by replacing protocatechuate 3,4-dioxygenase by the exogenous LigAB extradiol dioxygenase. Overexpression of pcaK encoding a transporter increased 8-fold 2,4 PDCA productivity from protocatechuate, reaching the highest value reported so far (0.58 g L-1h-1). Overexpression of the 4-hydroxybenzoate monooxygenase (pobA) speed up drastically the production of 2,4 PDCA from 4-hydroxybenzoate (0.056 g L-1h-1) or p-coumarate (0.012 g L-1h-1) achieving values 15-fold higher than those reported with Rhodococcus jostii biocatalysts. 2,4 PDCA was also bioproduced by using soda lignin as feedstock, paving the way for future polymeric lignin valorization approaches.

Keywords

2, 4 pyridine dicarboxylic acid; Bio-based plastics; Lignin; Pseudomonas putida; Resting cells.

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