2,4-PDCA

Name: 2,4-PDCA
Brand: MedChemExpress
SKU: HY-W017132
Rating: 4.5 (1 reviews)

Cat. No.: HY-W017132 Purity: 99.80%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2,4-PDCA (2,4 pyridine dicarboxylic acid) is a broad-spectrum inhibitor of 2OG oxygenase, including JmjC domain-containing family of histone demethylases (JHDMs). 2,4-PDCA is a target chemical in the field of bio-based plastics.

For research use only. We do not sell to patients.

2,4-PDCA Chemical Structure

CAS No. : 499-80-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 27	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Demethylase Isoform Specific Products:

View All Isoforms

KDM1/LSD1 KDM2 KDM3 KDM4 KDM5 KDM6 KDM7

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	GI₅₀	> 240 μM Compound: 2, PCA	Growth inhibition of human HeLa cells after 48 hrs by MTT assay Growth inhibition of human HeLa cells after 48 hrs by MTT assay	[PMID: 23964788]
KYSE-150 cell line	GI₅₀	> 240 μM Compound: 2, PCA	Growth inhibition of human KYSE-150 cells after 48 hrs by MTT assay Growth inhibition of human KYSE-150 cells after 48 hrs by MTT assay	[PMID: 23964788]
LNCaP	CC₅₀	588.7 μM Compound: 2	Cytotoxicity against human LNCAP cells after 3 days by MTT assay Cytotoxicity against human LNCAP cells after 3 days by MTT assay	[PMID: 24971742]
Sf21	IC₅₀	0.76 μM Compound: 1; 2,4-PDCA	Inhibition of KDM5A (1 to 797 residues) (unknown origin) expressed in insect sf21 cells using ARTK(Me3)QTARKSTGGKAPRK peptide/ 100 uM alpha-ketoglutarate as substrate/cofactor incubated for 45 mins by MALDI assay Inhibition of KDM5A (1 to 797 residues) (unknown origin) expressed in insect sf21 cells using ARTK(Me3)QTARKSTGGKAPRK peptide/ 100 uM alpha-ketoglutarate as substrate/cofactor incubated for 45 mins by MALDI assay	[PMID: 26699912]
Sf9	IC₅₀	1.5 μM Compound: 1, PCA	Inhibition of human recombinant PHD1 expressed in Sf9 cells by time-resolved fluorescence assay Inhibition of human recombinant PHD1 expressed in Sf9 cells by time-resolved fluorescence assay	[PMID: 20684604]
Sf9	IC₅₀	6.1 μM Compound: 1, PCA	Inhibition of human recombinant PHD2 expressed in Sf9 cells by time-resolved fluorescence assay Inhibition of human recombinant PHD2 expressed in Sf9 cells by time-resolved fluorescence assay	[PMID: 20684604]

In Vitro

2,4-PDCA (2 µM (tested in purified enzyme system), 25 µM (tested in cellular system)) significantly inhibits prolyl 4-hydroxylase activity and interferes with collagen synthesis^[3].
2,4-PDCA (700 µM) inhibits prolyl 4-hydroxylase activity and reduces procollagen production in the chicken calvaria model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

167.12

Formula

C₇H₅NO₄

CAS No.

499-80-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=NC=CC(C(O)=O)=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (598.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.9837 mL	29.9186 mL	59.8372 mL
5 mM	1.1967 mL	5.9837 mL	11.9674 mL
10 mM	0.5984 mL	2.9919 mL	5.9837 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB) HNMR (130 KB) LCMS (224 KB)

Handling Instructions (2659 KB)

References

[1]. Brewitz L, et al. Fluorinated derivatives of pyridine-2,4-dicarboxylate are potent inhibitors of human 2-oxoglutarate dependent oxygenases. J Fluor Chem. 2021 Jul;247:109804. [Content Brief]

[2]. Gómez-Álvarez H, et al. Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains. Bioresour Technol. 2022 Feb;346:126638. [Content Brief]

[3]. Hanauske-Abel HM. Prolyl 4-hydroxylase, a target enzyme for drug development. Design of suppressive agents and the in vitro effects of inhibitors and proinhibitors. J Hepatol. 1991;13 Suppl 3:S8-15; discussion S16. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.9837 mL	29.9186 mL	59.8372 mL	149.5931 mL
	5 mM	1.1967 mL	5.9837 mL	11.9674 mL	29.9186 mL
	10 mM	0.5984 mL	2.9919 mL	5.9837 mL	14.9593 mL
	15 mM	0.3989 mL	1.9946 mL	3.9891 mL	9.9729 mL
	20 mM	0.2992 mL	1.4959 mL	2.9919 mL	7.4797 mL
	25 mM	0.2393 mL	1.1967 mL	2.3935 mL	5.9837 mL
	30 mM	0.1995 mL	0.9973 mL	1.9946 mL	4.9864 mL
	40 mM	0.1496 mL	0.7480 mL	1.4959 mL	3.7398 mL
	50 mM	0.1197 mL	0.5984 mL	1.1967 mL	2.9919 mL
	60 mM	0.0997 mL	0.4986 mL	0.9973 mL	2.4932 mL
	80 mM	0.0748 mL	0.3740 mL	0.7480 mL	1.8699 mL
	100 mM	0.0598 mL	0.2992 mL	0.5984 mL	1.4959 mL

2,4-PDCA Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2,4-PDCA499-80-9Histone Demethylasebio-based plasticsJmjC domain-containing family of histone demethylasesJHDMsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

2,4-PDCA

Customer Review

2,4-PDCA Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Demethylase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

2,4-PDCA Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

2,4-PDCA Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report