2. Academic Validation
  3. Construction of developmentally inspired periosteum-like tissue for bone regeneration

Construction of developmentally inspired periosteum-like tissue for bone regeneration

  • Bone Res. 2022 Jan 3;10(1):1. doi: 10.1038/s41413-021-00166-w.
Kai Dai 1 2,  Shunshu Deng 1 2,  Yuanman Yu 1 2,  Fuwei Zhu 1 2,  Jing Wang 3 4,  Changsheng Liu 5 6 7
Affiliations

Affiliations

  • 1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, P. R. China.
  • 2 Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, P. R. China.
  • 3 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, P. R. China. wjbiomat@163.com.
  • 4 Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, P. R. China. wjbiomat@163.com.
  • 5 Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, P. R. China. liucs@ecust.edu.cn.
  • 6 Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, P. R. China. liucs@ecust.edu.cn.
  • 7 Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai, P. R. China. liucs@ecust.edu.cn.
PMID: 34975148 DOI: 10.1038/s41413-021-00166-w
Abstract

The periosteum, a highly vascularized thin tissue, has excellent osteogenic and bone regenerative abilities. The generation of periosteum-mimicking tissue has become a novel strategy for bone defect repair and regeneration, especially in critical-sized bone defects caused by trauma and bone tumor resection. Here, we utilized a bone morphogenetic protein-2 (BMP-2)-loaded scaffold to create periosteum-like tissue (PT) in vivo, mimicking the mesenchymal condensation during native long bone development. We found that BMP-2-induced endochondral ossification plays an indispensable role in the construction of PTs. Moreover, we confirmed that BMP-2-induced PTs exhibit a similar architecture to the periosteum and harbor abundant functional periosteum-like tissue-derived cells (PTDCs), blood vessels, and osteochondral progenitor cells. Interestingly, we found that the addition of chondroitin sulfate (CS), an essential component of the extracellular matrix (ECM), could further increase the abundance and enhance the function of recruited PTDCs from the PTs and finally increase the regenerative capacity of the PTs in autologous transplantation assays, even in old mice. This novel biomimetic strategy for generating PT through in vivo endochondral ossification deserves further clinical translation.

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