2. Academic Validation
  3. The First FRET-Based RNA Aptamer NanoKit for Sensitively and Specifically Detecting c-di-GMP

The First FRET-Based RNA Aptamer NanoKit for Sensitively and Specifically Detecting c-di-GMP

  • Nano Lett. 2022 Jan 26;22(2):716-725. doi: 10.1021/acs.nanolett.1c03970.
Ya Gao 1,  Yurui Xu 1,  Yanyan Li 2,  Kerong Chen 1,  Xiaotong Wu 1,  Yuhang Liu 1,  Xuli Feng 3,  Desheng Kong 2,  Xinghai Ning 1
Affiliations

Affiliations

  • 1 National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, Chemistry and Biomedicine Innovation Center, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing 210093, China.
  • 2 College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing 210093, China.
  • 3 School of Pharmaceutical Sciences and Innovative Drug Research Center, Chongqing University, Chongqing 401331, China.
PMID: 34994567 DOI: 10.1021/acs.nanolett.1c03970
Abstract

An effective method to identify c-di-GMP may significantly facilitate the exploration of its signaling pathways and Bacterial pathogenesis. Herein, we have developed the first conjugated polymer-amplified RNA aptamer NanoKit with a unique core-shell-shell architecture, which combines the advantages of high selectivity of RNA Aptamers and high sensitivity of strong fluorescence resonance energy transfer (FRET) effect, for precisely detecting c-di-GMP. We identified that NanoKit could selectively detect c-di-GMP with a low detection limit of 50 pM. Importantly, NanoKit could identify Bacterial species and physiological states, such as planktonic, biofilm, and even antibiotic-resistance, on the basis of their different c-di-GMP expression patterns. Particularly, NanoKit could distinguish Bacterial infection and inflammation and identify Pseudomonas aeruginosa associated pneumonia and sepsis, thereby guiding treatment choice and monitoring Antibiotic effects. Therefore, NanoKit provides a promising strategy to rapidly identify c-di-GMP and its associated diseases and may benefit for pathophoresis management.

Keywords

NanoKit; RNA aptamer; c-di-GMP; conjugated polymer; high sensitivity and specificity.

Figures
Products