2. Academic Validation
  3. Visual identification of gut bacteria and determination of natural inhibitors using a fluorescent probe selective for PGP-1

Visual identification of gut bacteria and determination of natural inhibitors using a fluorescent probe selective for PGP-1

  • Anal Chim Acta. 2022 Jan 25:1191:339280. doi: 10.1016/j.aca.2021.339280.
Chao Wang 1,  Zhenhao Tian 2,  Ming Zhang 3,  Ying Deng 4,  Xiangge Tian 5,  Lei Feng 6,  Jingnan Cui 7,  Tony D James 8,  Xiaochi Ma 9
Affiliations

Affiliations

  • 1 Second Affiliated Hospital, Dalian Medical University, Dalian, 116023, China; Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention, College of Integrative Medicine, College of Pharmacy, Dalian, 116044, China.
  • 2 School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, China.
  • 3 Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention, College of Integrative Medicine, College of Pharmacy, Dalian, 116044, China; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
  • 4 Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention, College of Integrative Medicine, College of Pharmacy, Dalian, 116044, China.
  • 5 Second Affiliated Hospital, Dalian Medical University, Dalian, 116023, China.
  • 6 Second Affiliated Hospital, Dalian Medical University, Dalian, 116023, China; School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, China. Electronic address: leifeng@dmu.edu.cn.
  • 7 State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
  • 8 School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, China; Department of Chemistry, University of Bath, Bath, BA2 7AY, UK. Electronic address: t.d.james@bath.ac.uk.
  • 9 Second Affiliated Hospital, Dalian Medical University, Dalian, 116023, China. Electronic address: maxc1978@163.com.
PMID: 35033245 DOI: 10.1016/j.aca.2021.339280
Abstract

PGP-1 is a Bacterial hydrolase that can hydrolyze the amide bond of the l-pyroglutamate (L-pGlu) residue at the amino terminus of proteins and Peptides. Guided by the biological function of PGP-1, an off-on NIR fluorescent probe DDPA was developed for the visual sensing of PGP-1 by conjugating pyroglutamic acid (recognition group) and DDAN (fluorophore). Using intestinal bacteria cultivation, eight bacteria strains with active PGP-1 were identified and cultivated efficiently using DDPA. In addition, three natural inhibitors against PGP-1 were isolated from the medical herb Psoralea corylifolia, which could be used to interfere with Bacterial metabolism in the gut. As such, the fluorescent probe DDPA provides an efficient method and potential tool for the investigation of intestinal microbiota.

Keywords

Fluorescent probe; High throughput screening; Intestinal bacteria; Near-infrared; PGP-1.

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