2. Academic Validation
  3. Dissection of the antibacterial mechanism of zinc oxide nanoparticles with manipulable nanoscale morphologies

Dissection of the antibacterial mechanism of zinc oxide nanoparticles with manipulable nanoscale morphologies

  • J Hazard Mater. 2022 May 15;430:128436. doi: 10.1016/j.jhazmat.2022.128436.
Xinyi Zhu 1,  Jun Wang 1,  Ling Cai 2,  Yuan Wu 3,  Minghui Ji 4,  Huijun Jiang 5,  Jin Chen 6
Affiliations

Affiliations

  • 1 Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
  • 2 School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
  • 3 Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, China.
  • 4 School of Nursing, Nanjing Medical University, Nanjing 211166, China.
  • 5 School of Pharmacy, Nanjing Medical University, 211166 Nanjing, China.
  • 6 Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China; Jiangsu Province Engineering Research Center of Antibody Drug, Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University, Nanjing 211166, China; The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 211166 Nanjing, China. Electronic address: jchen@njmu.edu.cn.
PMID: 35158241 DOI: 10.1016/j.jhazmat.2022.128436
Abstract

Despite the extensive uses of ZnO nanoparticles as promising antimicrobial agents to tackle the severe microbial infections, the systematic Antibacterial studies on ZnO nanoparticles with manipulable nanoscale morphologies at the genetic expression level remain ill-defined. In this study, via a controllable thermal decomposition, ZnO nanoparticles of different morphologies were facilely prepared. Additionally, the surface PEGylation of ZnO was conducted to obtain the nanoparticles of low biotoxicity. While all the prepared ZnO nanoparticles exhibited the significantly chemical activities, the pronounced Antibacterial effect of obtained ZnO nanoparticles was also identified, in which the ultra-small ones (~5 nm) showed the best performance. Moreover, the Antibacterial activities of ZnO nanoparticles were studied by Bacterial nucleic acid leakage, Alkaline Phosphatase, biofilm and Reactive Oxygen Species (ROS) assays. Furthermore, the transcriptome analysis of ZnO nanoparticles with different morphologies against Escherichia coli (E. coli) revealed the underlying Antibacterial mechanism involved the signal transduction, material transport, energy metabolism and other biological processes. Therefore, the cost-effective preparation of ZnO nanoparticles with distinct morphological features provides insights for the development of application specific Antibacterial agents.

Keywords

Antibacterial agent; Nanoparticles; Thermal decomposition method; Transcriptome analysis; ZnO.

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