2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Cell Proliferation and Cytotoxicity
  6. Cell Counting Kit-8

Cell Counting Kit-8 

Cat. No.: HY-K0301
Manual SDS

Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.

Cell Counting Kit-8
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1 mL USD 45 In-stock
5 mL USD 85 In-stock
30 mL USD 295 In-stock
120 mL USD 945 In-stock
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2631 Publications Citing Use of MCE Cell Counting Kit-8

Proliferation Assay
Cell Viability Assay

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Feb 7;9(2):177.  [Abstract]

    CCK8 assay. Proliferation of CAR T cells during expansion in response to repetitive stimulation with EGFR antigen.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2018 Apr;15(4):4129-4136.  [Abstract]

    The effect of TRPM8 knockdown on (A) LNCaP and (B) PC3 cells proliferation is measured by CCK 8 assay.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: J Mol Liq. 2023 Mar 2.

    CCK-8 (10 μM; 24 h) is used to detect the survival rate of L929 cells after treatment with different concentrations of DESs.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Mar 8;9(1):63.  [Abstract]

    CCK8 (1-2 h) assay-based detection of IC50 of gemcitabine in ICC cells.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2022 Jul 14;20(1):324.  [Abstract]

    CCK-8 (10 μM; 1-2 h) assay was used to assess the effect of huc-MSCs-derived exosomes (1, 10, 50, 100 µg/ml) on BV2 cell viability, and ns indicates no significance.

    • Description

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    Cell Counting Kit-8 (CCK-8) provides a tool for studying induction and inhibition of cell proliferation in any in vitro model. Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4- disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator.

    CCK-8 is a one-bottle solution, ready to use. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays.

    1. More Sensitive than MTT, MTS, or WST-1

    2. No Toxicity to Cell

    3. Simple Steps (No Thawing Necessary)

    4. Stable One Bottle Solution : 1 year at 4°C
    Storage
    4°C, 2 years Keep away from light for long-term storage
    Protocol

    Cell Number Determination

    1. Inoculate cell suspension (100 μL/well) in a 96-well plate. Pre-incubate the plate in a humidified incubator (e.g., at 37°C, 5% CO2).

    2. Add 10 μL of the CCK-8 solution to each well of the plate. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

    3. Incubate the plate for 1 - 4 hours in the incubator.

    4. Measure the absorbance at 450 nm using a microplate reader.

    Cell Proliferation and Cytotoxicity Assay

    1. Seed cells in a 96-well plate at a density of 104-105 cells/well in 100 μL of culture medium with or without compounds to be tested. Culture the cells in a CO2 incubator at 37°C for 24 hours.

    2. Add 10 μL of various concentrations of substances to be tested to the plate.

    3. Incubate the plate for an appropriate length of time (e.g., 6, 12, 24 or 48 hours) in the incubator.

    4. Add 10 μL of CCK-8 solution to each well of the plate using a repeating pipettor. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

    5. Incubate the plate for 1 - 4 hours in the incubator.

    6. Before reading the plate, it is important to mix gently on an orbital shaker for 1 minute to ensure homogeneous distribution of color.

    7. Measure the absorbance at 450 nm using a microplate reader.
    Components
    Cat. No. Product Name Package
    HY-K0301-1 mL Cell Counting Kit-8 1 mL
    HY-K0301-5mL Cell Counting Kit-8 5 mL
    HY-K0301-30 mL Cell Counting Kit-8 5 mL × 6
    HY-K0301-120 mL Cell Counting Kit-8 5 mL × 24
    Documentation
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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