2. Academic Validation
  3. Chemoenzymatic Labeling Pathogens Containing Terminal N-Acetylneuraminic Acid-α(2-3)-Galactose Glycans

Chemoenzymatic Labeling Pathogens Containing Terminal N-Acetylneuraminic Acid-α(2-3)-Galactose Glycans

  • ACS Infect Dis. 2022 Mar 11;8(3):657-664. doi: 10.1021/acsinfecdis.2c00011.
Qizheng Wu 1 2,  Jinfeng Ye 1,  Yicong Chao 2,  Shuchen Dong 3,  Min Niu 2,  Yaqian Wang 1,  Zhaoxi Liu 1,  Wang Chen 4,  Ningning Ge 5,  Shuhua Lu 5,  Peng George Wang 6,  Min Chen 1
Affiliations

Affiliations

  • 1 The State Key Laboratory of Microbial Technology and National Glycoengineering Research Center, Shandong University, Qingdao 266237, China.
  • 2 Medical Research Center, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining 272000, China.
  • 3 Department of Gastroenterology, Qingdao Municipal Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao 266000, China.
  • 4 Department of Neurology, Linyi People's Hospital, Linyi 276000, China.
  • 5 Department of Clinical Laboratory, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining 272000, China.
  • 6 School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
PMID: 35179863 DOI: 10.1021/acsinfecdis.2c00011
Abstract

The N-acetylneuraminic acid-α(2-3)-galactose epitope is often located at the nonreducing terminal ends of glycans on the envelopes of many pathogens, and it is believed that this structure mimics a host's oligosaccharide so as to circumvent and/or counteract the host's immune responses. A chemoenzymatic method for the rapid and sensitive detection of N-acetylneuraminic acid-α(2-3)-galactose has been built, so we planned to examine whether the chemoenzymatic method could be applied on the detection of N-acetylneuraminic acid-α(2-3)-galactose on pathogens. Our results revealed that the chemoenzymatic method was rapid and sensitive for labeling live or dead Gram-positive Streptococcus agalactiae A909 and Gram-negative Campylobacter jejuni MK104 with N-acetylneuraminic acid-α(2-3)-galactose. This study suggested that the chemoenzymatic method was a new strategy for labeling pathogens and had potential for the diagnosis of or therapeutics for pathogenic Infection.

Keywords

Campylobacter jejuni; Neu5Acα(2−3)Gal epitope; Streptococcus agalactiae; chemoenzymatic labeling.

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