2. Academic Validation
  3. Targeting ferroptosis suppresses osteocyte glucolipotoxicity and alleviates diabetic osteoporosis

Targeting ferroptosis suppresses osteocyte glucolipotoxicity and alleviates diabetic osteoporosis

  • Bone Res. 2022 Mar 9;10(1):26. doi: 10.1038/s41413-022-00198-w.
Yiqi Yang  # 1,  Yixuan Lin  # 1,  Minqi Wang 2,  Kai Yuan 1,  Qishan Wang 1,  Pei Mu 3,  Jingke Du 1,  Zhifeng Yu 1,  Shengbing Yang 1,  Kai Huang 1,  Yugang Wang 4,  Hanjun Li 5 6,  Tingting Tang 7
Affiliations

Affiliations

  • 1 Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • 2 Department of Bone and Joint Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  • 3 Department of Orthopaedics, Shanghai Jiangong Hospital, Shanghai, China.
  • 4 Department of Trauma Surgery, Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  • 5 Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. hanklee@sjtu.edu.cn.
  • 6 Clinical Stem Cell Research Center, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. hanklee@sjtu.edu.cn.
  • 7 Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ttt@sjtu.edu.cn.
  • # Contributed equally.
PMID: 35260560 DOI: 10.1038/s41413-022-00198-w
Abstract

Diabetic osteoporosis (DOP) is the leading complication continuously threatening the bone health of patients with diabetes. A key pathogenic factor in DOP is loss of osteocyte viability. However, the mechanism of osteocyte death remains unclear. Here, we identified Ferroptosis, which is iron-dependent programmed cell death, as a critical mechanism of osteocyte death in murine models of DOP. The diabetic microenvironment significantly enhanced osteocyte Ferroptosis in vitro, as shown by the substantial lipid peroxidation, iron overload, and aberrant activation of the Ferroptosis pathway. RNA Sequencing showed that heme oxygenase-1 (HO-1) expression was notably upregulated in ferroptotic osteocytes. Further findings revealed that HO-1 was essential for osteocyte Ferroptosis in DOP and that its promoter activity was controlled by the interaction between the upstream NRF2 and c-JUN transcription factors. Targeting Ferroptosis or HO-1 efficiently rescued osteocyte death in DOP by disrupting the vicious cycle between lipid peroxidation and HO-1 activation, eventually ameliorating trabecular deterioration. Our study provides insight into DOP pathogenesis, and our results provide a mechanism-based strategy for clinical DOP treatment.

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