2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis
  3. Reactive Oxygen Species Endogenous Metabolite Apoptosis
  4. Zinc Protoporphyrin

Zinc Protoporphyrin  (Synonyms: Zn(II)-protoporphyrin IX; ZnPP; Zinc Protoporphyrin-9)

Cat. No.: HY-101193 Purity: 99.87%
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Zinc Protoporphyrin (Zn(II)-protoporphyrin IX) is an orally active and competitive heme oxygenase-1 (HO-1) inhibitor and markedly attenuates the protective effects of Phloroglucinol (PG) against H2O2. Zinc Protoporphyrin is used as a screening marker of iron deficiency in individual pregnant women and children, but also to assess population iron status in combination with haemoglobin concentration. Zinc Protoporphyrin has anti-cancer activity.

For research use only. We do not sell to patients.

Zinc Protoporphyrin Chemical Structure

Zinc Protoporphyrin Chemical Structure

CAS No. : 15442-64-5

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10 mg USD 75 In-stock
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50 mg USD 210 In-stock
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Based on 39 publication(s) in Google Scholar

Other Forms of Zinc Protoporphyrin:

Zinc Protoporphyrin Related Antibodies

Top Publications Citing Use of Products

39 Publications Citing Use of MCE Zinc Protoporphyrin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Zinc Protoporphyrin (Zn(II)-protoporphyrin IX) is an orally active and competitive heme oxygenase-1 (HO-1) inhibitor and markedly attenuates the protective effects of Phloroglucinol (PG) against H2O2[1]. Zinc Protoporphyrin is used as a screening marker of iron deficiency in individual pregnant women and children, but also to assess population iron status in combination with haemoglobin concentration[2]. Zinc Protoporphyrin has anti-cancer activity[3].

IC50 & Target[1][2]

Human Endogenous Metabolite

 

In Vitro

to increase in Protoporphyrin (Zn(II)-protoporphyrin IX; 5 μM; 72 hours) causes the fraction of late apoptotic and necrotic cells increasing from 10.9% in controls to 30.4% after 72 h[3].
Zinc Protoporphyrin (1.25-40 μM; 48 or 72 hours) exerts cytostatic/cytotoxic effects against tumor cells[3].
Zinc Protoporphyrin (2.5, 5 μM; 48 or 72 hours) results in dose- and time-dependent reduction of cells in the G1 phase of the cell cycle[3].
Zinc Protoporphyrin (1.25-40 μM; 48 hours) leads to the accumulation of cleaved (active) caspase-3[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Zinc Protoporphyrin Related Antibodies

Apoptosis Analysis[3]

Cell Line: C-26 cells
Concentration: 5 μM
Incubation Time: 72 hours
Result: The fraction of late apoptotic and necrotic cells increased from 10.9% in controls to 30.4% after 72 h.

Cell Cytotoxicity Assay[3]

Cell Line: C-26 and MDA-MB231 cells
Concentration: 1.25, 2.5, 5, 10, 20, 40 μM
Incubation Time: 48 or 72 hours
Result: Exerted cystostatic/cytotoxic effects against tumor cells.

Cell Cycle Analysis[3]

Cell Line: C-26 cells
Concentration: 2.5, 5 μM
Incubation Time: 48 or 72 hours
Result: Resulted in dose- and time-dependent reduction of cells in G1 phase of the cell cycle.

Western Blot Analysis[3]

Cell Line: C-26 cells
Concentration: 1.25, 2.5, 5, 10, 20, 40 μM
Incubation Time: 48 hours
Result: Leaded to accumulation of cleaved (active) caspase-3.
In Vivo

Zinc Protoporphyrin (12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.; from day 7 to 19) exerts dose-dependent antitumor effects manifested by the retardation of tumor growth[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice inoculated with C-26 cells[3]
Dosage: 12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.
Administration: IP or PO; from day 7 to 19
Result: Exerted dose-dependent antitumor effects manifested by the retardation of tumor growth.
Molecular Weight

626.02

Formula

C34H32N4O4Zn
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

OC(CCC1=C(C=C2[N]3=C4C(C)=C2CCC(O)=O)[N-]([Zn+2]35[N]6=C7C=C(C(C=C)=C8C)[N-]5C8=C4)C(C=C6C(C=C)=C7C)=C1C)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 20.83 mg/mL (33.27 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5974 mL 7.9870 mL 15.9739 mL
5 mM 0.3195 mL 1.5974 mL 3.1948 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 1.67 mg/mL (2.67 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.87%

COA (259 KB) HNMR (270 KB) RP-HPLC (256 KB) MS (70 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5974 mL 7.9870 mL 15.9739 mL 39.9348 mL
5 mM 0.3195 mL 1.5974 mL 3.1948 mL 7.9870 mL
10 mM 0.1597 mL 0.7987 mL 1.5974 mL 3.9935 mL
15 mM 0.1065 mL 0.5325 mL 1.0649 mL 2.6623 mL
20 mM 0.0799 mL 0.3993 mL 0.7987 mL 1.9967 mL
25 mM 0.0639 mL 0.3195 mL 0.6390 mL 1.5974 mL
30 mM 0.0532 mL 0.2662 mL 0.5325 mL 1.3312 mL
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Zinc Protoporphyrin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Zinc Protoporphyrin15442-64-5Zn(II)-protoporphyrin IX ZnPP Zinc Protoporphyrin-9Zinc Protoporphyrin9Zinc Protoporphyrin 9Zinc Protoporphyrin-9Reactive Oxygen SpeciesEndogenous MetaboliteApoptosishemeoxygenase-1HO-1PhloroglucinolH2O2screeningmarkerirondeficiency,anti-cancerInhibitorinhibitorinhibit

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