2. Academic Validation
  3. Cell-wall damage activates DOF transcription factors to promote wound healing and tissue regeneration in Arabidopsis thaliana

Cell-wall damage activates DOF transcription factors to promote wound healing and tissue regeneration in Arabidopsis thaliana

  • Curr Biol. 2022 May 9;32(9):1883-1894.e7. doi: 10.1016/j.cub.2022.02.069.
Ai Zhang 1,  Keita Matsuoka 2,  Abdul Kareem 1,  Madalen Robert 3,  Pawel Roszak 4,  Bernhard Blob 4,  Anchal Bisht 5,  Lieven De Veylder 5,  Cătălin Voiniciuc 6,  Masashi Asahina 7,  Charles W Melnyk 8
Affiliations

Affiliations

  • 1 Department of Plant Biology, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, Almas allé 5, 756 51, Uppsala, Sweden.
  • 2 Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Japan.
  • 3 Independent Junior Research Group - Designer Glycans, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany.
  • 4 The Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, UK.
  • 5 Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium.
  • 6 Independent Junior Research Group - Designer Glycans, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany; Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.
  • 7 Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Japan; Advanced Instrumental Analysis Center, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Japan.
  • 8 Department of Plant Biology, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, Almas allé 5, 756 51, Uppsala, Sweden. Electronic address: charles.melnyk@slu.se.
PMID: 35320706 DOI: 10.1016/j.cub.2022.02.069
Abstract

Wound healing is a fundamental property of Plants and Animals that requires recognition of cellular damage to initiate regeneration. In Plants, wounding activates a defense response via the production of jasmonic acid and a regeneration response via the hormone Auxin and several ethylene response factor (ERF) and NAC domain-containing protein (ANAC) transcription factors. To better understand how Plants recognize damage and initiate healing, we searched for factors upregulated during the horticulturally relevant process of plant grafting and found four related DNA binding with one finger (DOF) transcription factors, HIGH CAMBIAL ACTIVITY2 (HCA2), TARGET OF MONOPTEROS6 (TMO6), DOF2.1, and DOF6, whose expression rapidly activated at the Arabidopsis graft junction. Grafting or wounding a quadruple hca2, tmo6, dof2.1, dof6 mutant inhibited vascular and cell-wall-related gene expression. Furthermore, the quadruple dof mutant reduced callus formation, tissue attachment, vascular regeneration, and pectin methylesterification in response to wounding. We also found that activation of DOF gene expression after wounding required Auxin, but hormone treatment alone was insufficient for their induction. However, modifying cell walls by enzymatic digestion of cellulose or pectin greatly enhanced TMO6 and HCA2 expression, whereas genetic modifications to the pectin or cellulose matrix using the PECTIN METHYLESTERASE INHIBITOR5 overexpression line or korrigan1 mutant altered TMO6 and HCA2 expression. Changes to the cellulose or pectin matrix were also sufficient to activate the wound-associated ERF115 and ANAC096 transcription factors, suggesting that cell-wall damage represents a common mechanism for wound perception and the promotion of tissue regeneration.

Keywords

Arabidopsis; DOF transcription factors; auxin; cell walls; cell-wall damage; grafting; regeneration; wound healing.

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