2. Academic Validation
  3. Metabonomics analysis of flavonoids in seeds and sprouts of two Chinese soybean cultivars

Metabonomics analysis of flavonoids in seeds and sprouts of two Chinese soybean cultivars

  • Sci Rep. 2022 Apr 1;12(1):5541. doi: 10.1038/s41598-022-09408-1.
Weiwei Bi # 1 2 3,  Guixing Zhao # 4,  Yutong Zhou 5,  Xiaoyu Xia 4,  Jinsheng Wang 4,  Guangjin Wang 4,  Shuwen Lu 6,  Wenjin He 4,  Taifei Bi 7,  Jinrong Li 4
Affiliations

Affiliations

  • 1 Heilongjiang Academy of Agricultural Sciences Post-Doctoral Station, Nangang District, Harbin, 150086, People's Republic of China. weiwebi5321@163.com.
  • 2 Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Nangang District, Harbin, 150086, People's Republic of China. weiwebi5321@163.com.
  • 3 Colleage of Life Sciences, Yulin University, Yuyang District, Yulin, 719000, People's Republic of China. weiwebi5321@163.com.
  • 4 Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Nangang District, Harbin, 150086, People's Republic of China.
  • 5 School of Hydraulic and Electric Power, Heilongjiang University, Nangang District, Harbin, 150086, People's Republic of China.
  • 6 Food Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Nangang District, Harbin, 150086, People's Republic of China.
  • 7 Colleage of Life Sciences, Yulin University, Yuyang District, Yulin, 719000, People's Republic of China.
  • # Contributed equally.
PMID: 35365712 DOI: 10.1038/s41598-022-09408-1
Abstract

A popular food in China, soybean seeds and sprouts contained many biologically active substances which are beneficial to the human body, such as Flavonoids. Northeast of China is the main producing area of soybean. The experimental Materials came from the main soybean producing areas in Northeast China, this study compared Flavonoids of two China cultivars of soybeans, Heinong52(HN52) and Heinong71(HN71). Here, we also considered the effects of germination on the chemical profile of Flavonoids. Using a LC-ESI-MS/MS system, 114 differential flavonoid metabolites were identified. A total of 18 metabolites were significantly different between the two soybean varieties before germination, of which 14 were up-regulated and 4 were down-regulated. After germination, 33 significantly different metabolites were found in the two soybean sprouts, of which 19 were up-regulated and 14 were down-regulated. These experimental results revealed significant up-regulation of metabolites in soybean sprouts compared with soybean seeds, thus suggesting that soybean germination may increase content of flavonoid metabolites. There are six main pathways for the synthesis of flavonoids: isoflavonoid biosynthesis, flavonoid biosynthesis, flavone and flavonol biosynthesis, biosynthesis of secondary metabolites, and biosynthesis of Phenylpropanoids. Soybean seeds lack flavone and flavanol biosynthesis and develop the capacity for this biosynthetic pathway after germination as sprouts. Isoflavonoid biosynthesis is the most abundantly utilized pathway.

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