2. Academic Validation
  3. Cryptosporidium parvum downregulates miR-181d in HCT-8 cells via the p50-dependent TLRs/NF-κB pathway

Cryptosporidium parvum downregulates miR-181d in HCT-8 cells via the p50-dependent TLRs/NF-κB pathway

  • Vet Parasitol. 2022 May;305:109710. doi: 10.1016/j.vetpar.2022.109710.
Ruiying Feng 1,  Ziwen Niu 1,  Xiaotian Zhang 1,  Wenyan Hou 1,  Yingying Zhang 1,  Fuchun Jian 1,  Changshen Ning 1,  Longxian Zhang 1,  Sumei Zhang 2,  Rongjun Wang 3
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450046, China.
  • 2 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450046, China. Electronic address: smzhang2815@henau.edu.cn.
  • 3 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450046, China. Electronic address: wrj-1978@henau.edu.cn.
PMID: 35462275 DOI: 10.1016/j.vetpar.2022.109710
Abstract

Cryptosporidium spp. can cause diarrhea and even death in humans and Animals. Host MicroRNAs (miRNAs) play an important role in the post-transcriptional regulation of the innate immune response to Cryptosporidium Infection. To study host miRNA activity in the innate immune response to C. parvum Infection, we examined the expression of miR-181d in HCT-8 cells infected with C. parvum and found that it was significantly downregulated, while TLR2, TLR4, NF-κB, and MyD88 involved in the TLR/NF-κB signaling pathway were significantly upregulated at the early stages of C. parvum Infection. We transfected cells with short-interfering RNAs (siRNA) as TLR2, TLR4, and NF-κB inhibitors. Analysis by quantitative real-time polymerase chain reaction (qPCR) and western blot confirmed that C. parvum downregulates miR-181d expression via the p50 subunit-dependent TLR2/TLR4-NF-κB signaling pathway in HCT-8 cells. This study provides a new theoretical foundation to elucidate the regulatory mechanism of host miRNAs against Cryptosporidium Infection.

Keywords

Cryptosporidium parvum; HCT-8; MiR-181d; NF-κB; TLRs.

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