2. Academic Validation
  3. Organelle-targeted imaging based on fluorogen-activating RNA aptamers in living cells

Organelle-targeted imaging based on fluorogen-activating RNA aptamers in living cells

  • Anal Chim Acta. 2022 May 29;1209:339816. doi: 10.1016/j.aca.2022.339816.
Yan Peng 1,  Xilei Ai 1,  Yi Yuan 2,  Juan Dong 2,  Xin Cui 2,  Feng Du 2,  Xin Huang 3,  Zhuo Tang 4
Affiliations

Affiliations

  • 1 Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, 610041, PR China; Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, PR China.
  • 2 Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, 610041, PR China.
  • 3 Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, 610041, PR China. Electronic address: huangxin@cib.ac.cn.
  • 4 Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, 610041, PR China. Electronic address: tangzhuo@cib.ac.cn.
PMID: 35569846 DOI: 10.1016/j.aca.2022.339816
Abstract

Organelles play specific and indispensable roles in cellular processes, and their dysfunction causes a series of diseases. Monitoring their variations is crucial to understanding their roles in physiopathology. Unlike most organelle imaging tools based on chemical probes or proteins, we developed a novel molecular tool for cell organelle imaging based on nucleic acids. Real-time imaging of mitochondria and nucleus in living cells was realized easily through using RNA-based tools, which are composed of Organelle-targeted RNA Sequence and Fluorogen-activating RNA aptamer. This genetically encoded Organelle-targeted RNA imaging system provides a powerful platform for measuring the properties of organelles and intracellular structures in living cells, due to its nontoxicity, low fluorescence background, ease of construction, and modular properties.

Keywords

Aptasensor; Imaging; Live cell; Organelle; RNA.

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