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  3. Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations

Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations

  • Nat Neurosci. 2022 Jun;25(6):805-817. doi: 10.1038/s41593-022-01073-x.
Zhao-Zhe Hao  # 1,  Jia-Ru Wei  # 1,  Dongchang Xiao  # 1,  Ruifeng Liu 1,  Nana Xu 1,  Lei Tang 1,  Mengyao Huang 1,  Yuhui Shen 1,  Changsheng Xing 2,  Wanjing Huang 1,  Xialin Liu 1,  Mengqing Xiang 3,  Yizhi Liu 4 5 6,  Zhichao Miao 7 8 9,  Sheng Liu 10 11
Affiliations

Affiliations

  • 1 State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China.
  • 2 Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
  • 3 State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China. xiangmq3@mail.sysu.edu.cn.
  • 4 State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China. yzliu62@yahoo.com.
  • 5 Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China. yzliu62@yahoo.com.
  • 6 Research Unit of Ocular Development and Regeneration, Chinese Academy of Medical Sciences, Beijing, China. yzliu62@yahoo.com.
  • 7 Translational Research Institute of Brain and Brain-Like Intelligence and Department of Anesthesiology, Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine, Shanghai, China. zmiao@ebi.ac.uk.
  • 8 European Bioinformatics Institute, European Molecular Biology Laboratory, Wellcome Genome Campus, Cambridge, UK. zmiao@ebi.ac.uk.
  • 9 Guangzhou Laboratory, Guangzhou International Bio Island, Guangzhou, China. zmiao@ebi.ac.uk.
  • 10 State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China. liush87@mail.sysu.edu.cn.
  • 11 Guangdong Province Key Laboratory of Brain Function and Disease, Guangzhou, China. liush87@mail.sysu.edu.cn.
  • # Contributed equally.
PMID: 35637371 DOI: 10.1038/s41593-022-01073-x
Abstract

The extent to which neurogenesis occurs in adult primates remains controversial. In this study, using an optimized single-cell RNA Sequencing pipeline, we profiled 207,785 cells from the adult macaque hippocampus and identified 34 cell populations comprising all major hippocampal cell types. Analysis of their gene expression, specification trajectories and gene regulatory networks revealed the presence of all key neurogenic precursor cell populations, including a heterogeneous pool of radial glia-like cells (RGLs), intermediate progenitor cells (IPCs) and neuroblasts. We identified HMGB2 as a novel IPC marker. Comparison with mouse single-cell transcriptomic data revealed differences in neurogenic processes between species. We confirmed that neurogenesis is recapitulated in ex vivo neurosphere cultures from adult primates, further supporting the existence of neural precursor cells (NPCs) that are able to proliferate and differentiate. Our large-scale dataset provides a comprehensive adult neurogenesis atlas for primates.

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