2. Academic Validation
  3. Closed-tube saltatory rolling circle amplification with hydroxynaphthol blue for visual on-site detection of peanut as an allergenic food

Closed-tube saltatory rolling circle amplification with hydroxynaphthol blue for visual on-site detection of peanut as an allergenic food

  • Food Chem. 2022 Nov 1;393:133408. doi: 10.1016/j.foodchem.2022.133408.
Qian Yang 1,  Haoyu Yang 2,  Ning Yuan 3,  Shuna Zuo 3,  Yunzhe Zhang 3,  Wei Zhang 4
Affiliations

Affiliations

  • 1 College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China; College of Public Health, Hebei University, Baoding 071002, China.
  • 2 Department of Physical Education, Hebei Agricultural University, Baoding 071001, China.
  • 3 College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
  • 4 College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China; College of Life Sciences, Hebei Agricultural University, Baoding 071001, China; Hebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism, Hebei Agricultural University, Baoding 071001, China. Electronic address: zhangwei19631126@126.com.
PMID: 35691067 DOI: 10.1016/j.foodchem.2022.133408
Abstract

Peanut allergy as one of the major causes of food-induced anaphylaxis is usually life-threatening for specific individuals. Nowadays, the effective remedy is avoidance of consuming allergen-containing food, urging monitoring the presence of peanut in foods. Herein, we proposed a closed-tube saltatory rolling circle amplification (SRCA) assay with hydroxynaphthol blue (HNB) for visual on-site detection of peanut allergen, displaying highly specificity for Ara h 1-encoding gene. The results can be discriminated by naked eye without opening the tube, averting costly sophisticated apparatus and reducing the risk of cross-contamination. The assay was 1000-, 100- and 10-fold more sensitive than PCR, LAMP and SRCA, respectively. The relative limit of detection is 0.01% in a binary mixture. The relative sensitivity, specificity and accuracy of this method were 100%, 97.50% and 98.67%, respectively. Thus, the visual closed-tube HNB-SRCA assay with superiorities of easy-to-perform, cost-effective and user-friendly has excellent potential for on-site application of food allergy.

Keywords

Food allergy; Peanut; Saltatory rolling circle amplification; Visual detection.

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