2. Academic Validation
  3. Antiaging effect of inotodiol on oxidative stress in human dermal fibroblasts

Antiaging effect of inotodiol on oxidative stress in human dermal fibroblasts

  • Biomed Pharmacother. 2022 Sep:153:113311. doi: 10.1016/j.biopha.2022.113311.
Seung Hoon Lee 1,  Gun-Woo Won 2,  Seung-Hyeon Choi 1,  Mi-Yoon Kim 3,  Cheong-Hae Oh 3,  Jong-Tae Park 4,  Jong-Il Park 5
Affiliations

Affiliations

  • 1 Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea; Translational Immunology Institute, Chungnam National University College of Medicine, Daejeon, Republic of Korea.
  • 2 Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea; Brain Korea 21 FOUR Project for Medical Science, Chungnam National University, Daejeon, Republic of Korea.
  • 3 Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
  • 4 Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea; CARBOEXPERT Inc., Daejeon 34134, Republic of Korea. Electronic address: jtpark@cnu.ac.kr.
  • 5 Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea; Translational Immunology Institute, Chungnam National University College of Medicine, Daejeon, Republic of Korea; Brain Korea 21 FOUR Project for Medical Science, Chungnam National University, Daejeon, Republic of Korea. Electronic address: jipark@cnu.ac.kr.
PMID: 35759867 DOI: 10.1016/j.biopha.2022.113311
Abstract

Oxidative damage is one of the major causes of human skin aging. Inotodiol is a lanostane triterpenoid that demonstrates Antiviral, Anticancer, and anti-inflammatory activities. Previous studies have reported that inotodiol also has antiallergic effects. However, whether inotodiol inhibits oxidative stress-induced human skin aging is not known. Stimulation of human dermal fibroblast cells with hydrogen peroxide is related to skin aging. Inotodiol inhibited the expression of mitogen-activated protein kinase (MAPK) and NADPH Oxidase 5 (NOX5). Moreover, inotodiol effectively decreased nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), as well as nitric oxide (NO), Reactive Oxygen Species (ROS), cyclooxygenase-2 (COX-2), and cytokines such as IL-1β, IL-6, and TNF-α. Based on our results, inotodiol protects human dermal fibroblast by preventing MAPK-NOX5 and NF-κB activation and attenuates the expression of aging genes. Inotodiol may therefore be considered a potential candidate for developing natural antiaging products, because it protects the human skin from oxidative stress-induced skin aging by inhibiting the MAPK-NOX5 and NF-κB signaling pathways.

Keywords

Human dermal fibroblats; Inotodiol; MAPK; NADPH Oxidase 5; Skin aging.

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