Inotodiol 

Inotodiol activates p53 signaling pathway, inhibits MMP-2/9, and exhibits antitumor activity in cancer cell HeLa. Inotodiol inhibits the generation of ROS and exhibits antioxidant and neuroprotective effects. Inotodiol inhibits the activation of MAPK and NF-κB signaling pathway, and exhibits anti-inflammatory activity. Inotodiol inhibits TLR-4 mediated TNF-α production (IC₅₀s in BMMC and BMDM is 0.7 μM and 3.0 μM), inhibits the degranulation in mast cell, exhibits anti-allergic activity. Inotodiol is orally active^{[1][2][3][4][5]}.

Inotodiol (0-100 μM, 72 h) inhibits the proliferation of cell HeLa, inhibits the cell migration and invasion, induces apoptosis i HeLa^[2].
Inotodiol (0.1-10 μM, 28 h) inhibits the ROS generation, activates SOD, improves oxygen and glucose deprivation-induced mitochondrial dysfunction, reduces the leakage of lactate dehydrogenase and exhibits neuroprotective effect in PC12 cell^[3].
Inotodiol (2 μg/mL, 24 h) inhibits H₂O₂-induced expression of IL-1β, IL-6, TNF-α and COX-2, and blocks the senescence in human dermal fibroblasts (HDF)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Inotodiol (2-20 mpk, po, once daily for 3-5 days) inhibits allergic reactions in passive systemic anaphylaxis and passive cutaneous anaphylaxis, exhibits antiasthmatic activity in mouse models^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

442.72

C₃₀H₅₀O₂

35963-37-2

Solid

White to off-white

C[C@@]1(CC[C@@H]2O)C(CC[C@@]34C)=C(CC[C@@]1([H])C2(C)C)[C@@]3(CC[C@]4([H])[C@H](C)[C@H](O)C/C=C(C)\C)C

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Nakata T, et al. Structure determination of inonotsuoxides A and B and in vivo anti-tumor promoting activity of inotodiol from the sclerotia of Inonotus obliquus. Bioorg Med Chem. 2007;15(1):257-264.  [Content Brief]

  [2]. Zhang SD, et al., Inotodiol inhibits cells migration and invasion and induces apoptosis via p53-dependent pathway in HeLa cells. Phytomedicine. 2019 Jul;60:152957.  [Content Brief]

  [3]. Li Y, et al. Inotodiol protects PC12 cells against injury induced by oxygen and glucose deprivation/restoration through inhibiting oxidative stress and apoptosis[J]. Journal of Applied Biomedicine, 2018, 16(2): 126-132.

  [4]. Lee SH, et al., Antiaging effect of inotodiol on oxidative stress in human dermal fibroblasts. Biomed Pharmacother. 2022 Sep;153:113311.  [Content Brief]

  [5]. Liu Y, et al., Inotodiol, an antiasthmatic agent with efficacy and safety, preferentially impairs membrane-proximal signaling for mast cell activation. Int Immunopharmacol. 2023 Apr;117:109854.  [Content Brief]