2. Academic Validation
  3. Identification of lysine-lactylated substrates in gastric cancer cells

Identification of lysine-lactylated substrates in gastric cancer cells

  • iScience. 2022 Jun 17;25(7):104630. doi: 10.1016/j.isci.2022.104630.
Dawei Yang 1,  Jie Yin 2,  Liuqun Shan 3,  Xingling Yi 4,  Wei Zhang 5,  Yongbin Ding 6 3
Affiliations

Affiliations

  • 1 Department of Pharmacy, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210003, China.
  • 2 Department of General Surgery, Haian People's Hospital, Haian, Jiangsu 226600, China.
  • 3 Department of General Surgery, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210003, China.
  • 4 Micron Biotechnology Co., Ltd., Hangzhou 310051, China.
  • 5 Department of General Surgery, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
  • 6 Department of General Surgery, Pukou Branch Hospital of Jiangsu Province Hospital (Nanjing Pukou Central Hospital), Nanjing, Jiangsu 211800, China.
PMID: 35800753 DOI: 10.1016/j.isci.2022.104630
Abstract

Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene expression. However, Kla in non-histone mammalian proteins is unclear. Here, a comprehensive analysis of lactylated proteins in gastric Cancer AGS cells was conducted. There were 2375 Kla sites found in 1014 proteins. Interestingly, KEGG pathway analysis showed that these proteins were significantly enriched in spliceosome function. In addition, Kla was more abundant in gastric tumors than in adjacent tissues, and high levels of Kla in gastric tumors were associated with poor prognosis. These results suggest that Kla could be a prognostic marker in gastric Cancer. This lysine lactylome analysis in gastric Cancer cells, the first of its kind, provides a valuable foundation for further studies of Kla.

Keywords

Cancer; Cancer systems biology; Integrative aspects of cell biology; Molecular biology.

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