1. Academic Validation
  2. Ascorbate oxidase enabling glucometer readout for portable detection of hydrogen peroxide

Ascorbate oxidase enabling glucometer readout for portable detection of hydrogen peroxide

  • Enzyme Microb Technol. 2022 Oct:160:110096. doi: 10.1016/j.enzmictec.2022.110096.
Tao Tian 1 Hao Zhang 2 Feng-Qing Yang 3
Affiliations

Affiliations

  • 1 Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
  • 2 Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China. Electronic address: zhanghao199392@163.com.
  • 3 School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China. Electronic address: fengqingyang@cqu.edu.cn.
Abstract

A rapid, portable, and cost-effective method using personal glucose meter (PGM) for quantitative analysis of hydrogen peroxide (H2O2) was established based on ascorbate oxidase (AAO)-catalyzed reaction for the first time. Ascorbic acid (AA) can rapidly reduce ferricyanide (K3[Fe(CN)6]) to ferrocyanide (K4[Fe(CN)6]) in the glucose test strip and transfer electron to the electrode to generating a PGM detectable signal. Thus, the concentration of AA can be directly determined by the PGM as simple as measuring the blood glucose. On the other hand, AAO can catalyze the reduction of H2O2 and produce an enzyme-peroxide complex, which decreases the yields of dehydroascorbic acid formed by the oxidation of AA, resulting in the increase in PGM detectable signal of residual ascorbic acid (re-AA). Therefore, the concentration of H2O2 is proportional to the concentration of re-AA. After optimization of the experimental conditions, the developed method can be used to detect H2O2 at linear range of 2.5-5 × 103 μM with the quantification limit of 2.5 μM. In addition, the satisfactory spiked recoveries (95.3-108.9 %) of real samples (i.e., tap water, contact lens solution, medical hydrogen peroxide, and normal human serum) confirm its feasibility for practical applications. In short, this study provides a feasible PGM-based method for H2O2 detection with simple operations.

Keywords

Ascorbate oxidase; Ascorbic acid; Hydrogen peroxide; Personal glucose meter; Point-of-care testing.

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