1. Academic Validation
  2. Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress

Protective Effect of Resveratrol against Hypoxia-Induced Neural Oxidative Stress

  • J Pers Med. 2022 Jul 23;12(8):1202. doi: 10.3390/jpm12081202.
Amogh Auti 1 Nicola Alessio 2 Andrea Ballini 1 Mario Dioguardi 3 Stefania Cantore 4 Salvatore Scacco 5 Antonio Vitiello 6 Lucio Quagliuolo 1 Barbara Rinaldi 2 Luigi Santacroce 7 Marina Di Domenico 1 8 Mariarosaria Boccellino 1
Affiliations

Affiliations

  • 1 Department of Precision Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
  • 2 Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
  • 3 Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy.
  • 4 Independent Researcher, 70129 Bari, Italy.
  • 5 Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari ALDO MORO, 70124 Bari, Italy.
  • 6 Pharmaceutical Department, Local Sanitary Unit Umbria 1, 06132 Perugia, Italy.
  • 7 Microbiology and Virology Unit, Department of Interdisciplinary Medicine, School of Medicine, University of Bari ALDO MORO, 70124 Bari, Italy.
  • 8 Department of Biology, College of Science and Technology, Temple University, Philadelphia, PA 19122-6078, USA.
Abstract

Oxidative stress plays an important role in brain aging and in neurodegenerative diseases. New therapeutic agents are necessary to cross the blood-brain barrier and target disease pathogenesis without causing disagreeable side effects. Resveratrol (RSV) may act as a neuroprotective compound, but little is known about its potential in improving the cognitive and metabolic aspects that are associated with neurodegenerative diseases. The objective of this study was to investigate the protective effects and the underlying mechanisms of RSV against hypoxia-induced oxidative stress in neuronal PC12 cells. For the induction of the hypoxia model, the cells were exposed to oxygen-deprived gas in a hypoxic chamber. Cell cycle and Apoptosis were analyzed by a fluorescence activated cell sorting (FACS) analysis. The intracellular Reactive Oxygen Species (ROS) level was analyzed by using dichlorodihydrofluorescein diacetate (DCFDA) and 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) tests. The expression of activated Caspase-3, -9, Bcl-2, Bax, p53, and SOD was investigated by a Western blot analysis. We found that hypoxia reduced PC12 viability by inducing Apoptosis, while RSV treatment attenuated the ROS-induced damage by reducing Caspase-3, -9, and the Bax/Bcl-2 ratio. The RSV treated groups were found to improve cellular health, with a 7.41% increase in the S phase population in the 10 µM group, compared to the control. Hence, RSV has a protective effect in neuronal cells and may halt the cell cycle in the G1/S phase to repair the intracellular damage. Therefore, RSV could be a good candidate to act as an antioxidant and promising preventive therapeutic agent in neurodegenerative diseases for personalized medicine.

Keywords

PC12 cells; hypoxia; ischemia; oxidative stress; personalized medicine; resveratrol; translational research.

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