2. Academic Validation
  3. Hypertension secondary to nitric oxide depletion produces oxidative imbalance and inflammatory/fibrotic outcomes in the cornea of C57BL/6 mice

Hypertension secondary to nitric oxide depletion produces oxidative imbalance and inflammatory/fibrotic outcomes in the cornea of C57BL/6 mice

  • J Physiol Biochem. 2022 Aug 9. doi: 10.1007/s13105-022-00916-2.
Álvaro Santana-Garrido 1 2 3,  Claudia Reyes-Goya 1,  Ana Arroyo-Barrios 1,  Helder André 3,  Carmen M Vázquez 4 5 6,  Alfonso Mate 7 8
Affiliations

Affiliations

  • 1 Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, 41012, Seville, Spain.
  • 2 Epidemiología Clínica y Riesgo Cardiovascular, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013, Seville, Spain.
  • 3 Department of Clinical Neuroscience, St. Erik Eye Hospital, Karolinska Institutet, 11282, Stockholm, Sweden.
  • 4 Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, 41012, Seville, Spain. vazquez@us.es.
  • 5 Epidemiología Clínica y Riesgo Cardiovascular, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013, Seville, Spain. vazquez@us.es.
  • 6 Department of Clinical Neuroscience, St. Erik Eye Hospital, Karolinska Institutet, 11282, Stockholm, Sweden. vazquez@us.es.
  • 7 Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, 41012, Seville, Spain. mate@us.es.
  • 8 Epidemiología Clínica y Riesgo Cardiovascular, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/Consejo Superior de Investigaciones Científicas/Universidad de Sevilla, 41013, Seville, Spain. mate@us.es.
PMID: 35943663 DOI: 10.1007/s13105-022-00916-2
Abstract

Arterial hypertension (AH) leads to oxidative and inflammatory imbalance that contribute to fibrosis development in many target organs. Here, we aimed to highlight the harmful effects of severe AH in the cornea. Our experimental model was established by administration of NG-nitro-L-arginine-methyl-ester (L-NAME) to C57BL/6 mice, which were monitored weekly for arterial blood pressure and intraocular pressure (IOP). Morphological studies of ocular tissues were accompanied by analyses of Reactive Oxygen Species generation, and localization/expression of NAPDH oxidase isoforms (NOX1, NOX2, NOX4) and inflammatory biomarkers (PPARα, PPARγ, IL-1β, IL-6, IL-10, TNF-α, and COX-2). Masson's trichrome and Sirius Red staining were used to explore the fibrotic status of the cornea. The expression of collagen isoforms (COL1α1, COL1α2, COL3α1, COL4α1, COL4α2) and relevant metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) were also quantified to evaluate the participation of collagen metabolism in AH-related corneal damage. Hypertensive Animals showed an increase in IOP values, and a thinner cornea compared with normotensive controls. Moreover, AH increased NADPH Oxidase activity and Reactive Oxygen Species generation in the cornea, which was accompanied by transcriptional upregulation of NOX isoforms and inflammatory biomarkers, while reducing PPAR expression. L-NAME-treated Animals also developed corneal fibrosis with overexpression of collagen isoforms and reduction of factors responsible for collagen degradation. This is the first study reporting structural changes in the cornea and elevated IOP in L-NAME-treated mice. Overexpression of the NADPH Oxidase system and collagen deposition might play a substantial role in the pathogenic mechanisms contributing to ocular disturbances in a context of severe hypertension.

Keywords

Cornea; Fibrosis; Inflammation; Intraocular pressure; L-NAME hypertension; Oxidative stress.

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