2. Academic Validation
  3. ACOT7 protects epidermal stem cells against lipid peroxidation

ACOT7 protects epidermal stem cells against lipid peroxidation

  • In Vitro Cell Dev Biol Anim. 2022 Aug;58(7):549-557. doi: 10.1007/s11626-022-00703-9.
Guang Zhang 1 2,  Jiaxu Ma 1 2,  Zhenjie Wu 1 2,  Guoqi Cao 1 2,  Chunyan Liu 2 3,  Ru Song 1 2,  Rui Sun 1 2,  Aoyu Chen 2 3,  Yibing Wang 4 5 6,  Siyuan Yin 1 2 3
Affiliations

Affiliations

  • 1 Department of Plastic Surgery, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, People's Republic of China.
  • 2 Jinan Clinical Research Center for Tissue Engineering Skin Regeneration and Wound Repair, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, 250014, People's Republic of China.
  • 3 Department of Plastic Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, 250014, People's Republic of China.
  • 4 Department of Plastic Surgery, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, People's Republic of China. ybwang@sdfmu.edu.cn.
  • 5 Jinan Clinical Research Center for Tissue Engineering Skin Regeneration and Wound Repair, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, 250014, People's Republic of China. ybwang@sdfmu.edu.cn.
  • 6 Department of Plastic Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, 250014, People's Republic of China. ybwang@sdfmu.edu.cn.
PMID: 36036847 DOI: 10.1007/s11626-022-00703-9
Abstract

Epidermal stem cells (ESCs) are critical for skin regeneration and repair. Previous studies have shown that ESCs are susceptible to oxidative stress, which in turn leads to lipid peroxidation and affects skin repair. Our study aims to explore how ESCs resist lipid peroxidation. By performing proteomics analysis, we found that the expression of Acyl-CoA thioesterase 7 (ACOT7) was positively correlated with the concentration of transferrin. Overexpression adenovirus vectors of ACOT7 were constructed and transfected into ESCs. Levels of lipid peroxidation by flow cytometry, cell viabilities, and MDA levels were measured. The results revealed that ACOT7 could inhibit lipid peroxidation, reduce the level of malondialdehyde (MDA), and improve the survival rate of ESCs induced by H2O2, Erastin, and RSL3. Our data suggest that ACOT7 has an effect on protecting ESCs against iron-dependent lipid peroxidation.

Keywords

ACOT7; Epidermal stem cells; Lipid peroxidation; Transferrin.

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