2. Academic Validation
  3. RhoB affects colitis through modulating cell signaling and intestinal microbiome

RhoB affects colitis through modulating cell signaling and intestinal microbiome

  • Microbiome. 2022 Sep 16;10(1):149. doi: 10.1186/s40168-022-01347-3.
Jianming Yang  # 1,  Geng Pei  # 1,  Xuan Sun 1,  Yawen Xiao 1,  Chunhui Miao 1,  Lu Zhou 2,  Bangmao Wang 2,  Liu Yang 1,  Mingyu Yu 1,  Zhi-Song Zhang 3,  Evan T Keller 4,  Zhi Yao 1 5,  Quan Wang 6 7
Affiliations

Affiliations

  • 1 Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China.
  • 2 Department of Gastroenterology and Hepatology, Tianjin Medical University general hospital, Tianjin Medical University, Tianjin, 300070, China.
  • 3 State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Collaborative Innovation Center for Biotherapy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300350, China.
  • 4 Department of Urology, Biointerfaces Institute, University of Michigan, Ann Arbor, MI, USA.
  • 5 The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, Tianjin, 300070, China.
  • 6 Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, Tianjin Institute of Urology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 22 Qixiangtai Road, Heping District 300070, Tianjin, 300070, China. wangquan@tmu.edu.cn.
  • 7 The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Medical University, Tianjin, 300070, China. wangquan@tmu.edu.cn.
  • # Contributed equally.
PMID: 36114582 DOI: 10.1186/s40168-022-01347-3
Abstract

Background: The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed. RhoB regulates multiple cell functions; however, its role in colitis is unexplored.

Results: Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis. Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation. Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway. Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.

Conclusions: Taken together, a higher level of RhoB is associated with UC, which also contributes to UC development through modulating cell signaling and altering intestinal Bacterial composition and metabolites. These observations suggest that RhoB has potential as a biomarker and a treatment target for UC. Video Abstract.

Keywords

Inflammatory bowel diseases; Microbiome; RhoB; SCFAs.

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