1. Academic Validation
  2. Sinomenine Hydrochloride Promotes TSHR-Dependent Redifferentiation in Papillary Thyroid Cancer

Sinomenine Hydrochloride Promotes TSHR-Dependent Redifferentiation in Papillary Thyroid Cancer

  • Int J Mol Sci. 2022 Sep 14;23(18):10709. doi: 10.3390/ijms231810709.
Jing Zhang 1 Aomei Zhao 1 Xi Jia 1 Xinru Li 1 Yiqian Liang 1 Yan Liu 1 Xin Xie 1 Xijie Qu 1 Qi Wang 1 Yuemin Zhang 1 Rui Gao 1 Yan Yu 2 Aimin Yang 1
Affiliations

Affiliations

  • 1 Department of Nuclear Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
  • 2 Department of Public Health, Health Science Center of Xi'an Jiaotong University, Xi'an 710061, China.
Abstract

Radioactive iodine (RAI) plays an important role in the diagnosis and treatment of papillary thyroid Cancer (PTC). The curative effects of RAI therapy are not only related to radiosensitivity but also closely related to the accumulation of radionuclides in the lesion in PTC. Sinomenine hydrochloride (SH) can suppress tumor growth and increase radiosensitivity in several tumor cells, including PTC. The aim of this research was to investigate the therapeutic potential of SH on PTC cell redifferentiation. In this study, we treated BCPAP and TPC-1 cells with SH and tested the expression of thyroid differentiation-related genes. RAI uptake caused by SH-pretreatment was also evaluated. The results indicate that 4 mM SH significantly inhibited proliferation and increased the expression of the thyroid iodine-handling gene compared with the control group (p < 0.005), including the sodium/iodide symporter (NIS). Furthermore, SH also upregulated the membrane localization of NIS and RAI uptake. We further verified that upregulation of NIS was associated with the activation of the thyroid-stimulating hormone receptor (TSHR)/cyclic adenosine monophosphate (cAMP) signaling pathway. In conclusion, SH can inhibit proliferation, induce Apoptosis, promote redifferentiation, and then increase the efficacy of RAI therapy in PTC cells. Thus, our results suggest that SH could be useful as an Adjuvant therapy in combination with RAI therapy in PTC.

Keywords

papillary thyroid cancer; radioactive iodine uptake; redifferentiation; sinomenine hydrochloride; sodium/iodide symporter.

Figures
Products